{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:31:28Z","timestamp":1777930288509,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2004,5,1]],"date-time":"2004-05-01T00:00:00Z","timestamp":1083369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p>\n                    G\n                    <jats:sub>M1<\/jats:sub>\n                    - and G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidoses are lethal lysosomal diseases that are caused by a defect of acid hydrolases, resulting in the intralysosomal accumulation of the specific physiological substrates, G\n                    <jats:sub>M1<\/jats:sub>\n                    - and G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosides, respectively. In the present study a method for the diagnosis of canine G\n                    <jats:sub>M1<\/jats:sub>\n                    -gangliosidosis was established using canine cerebrospinal fluid (CSF). The concentration of G\n                    <jats:sub>M1<\/jats:sub>\n                    -ganglioside in CSF was determined by thin-layer chromatography\u2013enzyme immunostaining using biotin-conjugated cholera toxin B, which specifically binds with G\n                    <jats:sub>M1<\/jats:sub>\n                    -ganglioside. The concentration of CSF G\n                    <jats:sub>M1<\/jats:sub>\n                    -ganglioside was increased in Shiba dogs with G\n                    <jats:sub>M1<\/jats:sub>\n                    -gangliosidosis, and the increased level was approximately proportional to the age of the dogs. The concentration was high in the affected dog even at 5 months of age, when Shiba dogs with G\n                    <jats:sub>M1<\/jats:sub>\n                    -gangliosidosis first manifest neurologic signs. In addition, the concentration of CSF G\n                    <jats:sub>M1<\/jats:sub>\n                    -ganglioside in a dog with the G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis 0 variant (Sandhoff disease) was also 7 times the normal level. From these results it was concluded that this laboratory technique enables a definitive and early diagnosis of canine G\n                    <jats:sub>M1<\/jats:sub>\n                    -gangliosidosis even if tissues and organs cannot be obtained. However, because G\n                    <jats:sub>M1<\/jats:sub>\n                    -ganglioside can also be elevated in cases of G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis, it is necessary to assay for specific enzyme deficiencies to definitively separate G\n                    <jats:sub>M1<\/jats:sub>\n                    - from G\n                    <jats:sub>M2<\/jats:sub>\n                    -gangliosidosis.\n                  <\/jats:p>","DOI":"10.1177\/104063870401600308","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:26:52Z","timestamp":1305217612000},"page":"223-226","source":"Crossref","is-referenced-by-count":14,"title":["Increased Concentration of G\n                    <sub>M1<\/sub>\n                    -Ganglioside in Cerebrospinal Fluid in Dogs with G\n                    <sub>M1<\/sub>\n                    - and G\n                    <sub>M2<\/sub>\n                    -Gangliosidoses and its Clinical Application for Diagnosis"],"prefix":"10.1177","volume":"16","author":[{"given":"Hiroyuki","family":"Satoh","sequence":"first","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Yamato","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoya","family":"Asano","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Maede","sequence":"additional","affiliation":[{"name":"Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060\u20130818, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2004,5,1]]},"reference":[{"key":"bibr1-104063870401600308","first-page":"3827","volume-title":"The metabolic and molecular bases of inherited disease","author":"Gravel 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Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870401600308","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870401600308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:09:27Z","timestamp":1777680567000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870401600308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1177\/104063870401600308"],"URL":"https:\/\/doi.org\/10.1177\/104063870401600308","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}