{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:38:38Z","timestamp":1792726718849,"version":"4.5.0"},"reference-count":20,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:p>Six RNA extraction methods, i.e., RNAqueous kit, Micro-to-midi total RNA purification system, NucleoSpin RNA II, GenElute mammalian total RNA kit, RNeasy mini kit, and TRIzol LS reagent, were evaluated on blood and 7 tissues from pig infected with classical swine fever virus (CSFV). Each of the 6 extraction methods yielded sufficient RNA for positive results in a real-time reverse transcription\u2013PCR (RT-PCR) for CSFV, and all RNA, except the one extracted from blood by TRIzol LS reagent, yielded positive results in both a conventional RT-PCR for CSFV and a conventional RT-PCR for an endogenous gene encoding \u03b2-actin. The RNA extracted from blood by TRIzol LS reagent became positive in both conventional RT-PCR assays when it was diluted to 1:2, 1:4, or up to 1:64 in nuclease-free water. It is concluded that all 6 methods are more or less useful for the detection of CSFV by real-time and conventional RT-PCR in swine blood and tissues. However, some of the 6 reagents offer certain advantages not common to all 6 extraction procedures. For example, RNA extracted by the TRIzol LS reagent constantly had the highest yield; that by the RNAqueous kit had the highest A260\/A280 ratio for almost all samples; and that by the NucleoSpin RNA II and the GenElute mammalian total RNA kit was most likely to be free of contaminations with genomic DNA.<\/jats:p>","DOI":"10.1177\/104063870501700609","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:26:52Z","timestamp":1305217612000},"page":"574-578","source":"Crossref","is-referenced-by-count":61,"title":["Comparison of Six RNA Extraction Methods for the Detection of Classical Swine Fever Virus by Real-Time and Conventional Reverse Transcription\u2013PCR"],"prefix":"10.1177","volume":"17","author":[{"given":"Ming Y.","family":"Deng","sequence":"first","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Veterinary Service Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, Unites States Department of Agriculture, Greenport, NY 11944"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He","family":"Wang","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Veterinary Service Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, Unites States Department of Agriculture, Greenport, NY 11944"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon B.","family":"Ward","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Veterinary Service Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, Unites States Department of Agriculture, Greenport, NY 11944"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tammy R.","family":"Beckham","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Veterinary Service Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, Unites States Department of Agriculture, Greenport, NY 11944"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"McKenna","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Veterinary Service Laboratory, Veterinary Services, Animal and Plant Health Inspection Service, Unites States Department of Agriculture, Greenport, NY 11944"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2005,11,1]]},"reference":[{"key":"bibr1-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.485-493.2001"},{"key":"bibr2-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"bibr3-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1996.d01-332.x"},{"key":"bibr4-104063870501700609","first-page":"15","volume-title":"Current protocols in molecular biology","author":"Donald MC","year":"1984"},{"key":"bibr5-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.841-843.2004"},{"key":"bibr6-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.8.4.1775"},{"key":"bibr7-104063870501700609","first-page":"441","volume-title":"Veterinary virology","author":"Fenner FJ","year":"1993","edition":"2"},{"key":"bibr8-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1177\/104063870401600207"},{"key":"bibr9-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.31.3.565-568.1993"},{"key":"bibr10-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.5.10.2720"},{"key":"bibr11-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.6.1759"},{"key":"bibr12-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00142-5"},{"key":"bibr13-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00264-9"},{"key":"bibr14-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1039\/b304549h"},{"key":"bibr15-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.3.10.1783"},{"key":"bibr16-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.500-505.2003"},{"key":"bibr17-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1111\/j.1526-0968.2004.00161.x"},{"key":"bibr18-104063870501700609","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.04.014"},{"key":"bibr19-104063870501700609","unstructured":"Wolff A, Perch-Nielson IR, Poulsen CR, 2003, Removal of PCR inhibitors using dielectrophoresis for sample preparation in a microfabricated system. 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