{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:24:30Z","timestamp":1790555070937,"version":"4.1.0"},"reference-count":20,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>Studies were carried out to determine the cause of death in a prematurely born Thoroughbred foal that died 24 hours after birth. Necropsy revealed gross lesions suggestive of septicemia. A commercial Leptospira polymerase chain reaction (PCR) assay designed to specifically amplify the hemolysis-associated protein 1 ( hap1) gene present only in pathogenic Leptospira strains detected the presence of Leptospira DNA in various tissues of the foal. Histologic examination of lung, liver, kidney, and myocardium revealed numerous spirochetes in Warthin\u2013Starry-stained tissue sections. Results of PCR analysis and histologic examination suggested a leptospiral infection in the newborn foal. At the moment of death, the infection coexisted with a streptococcal-associated aspiration bronchopneumonia and postpartum septicemia. These findings indicate that the PCR assay based on the amplification of the hap1 gene represents a useful tool for specific detection of pathogenic leptospira in field samples taken from horses.<\/jats:p>","DOI":"10.1177\/104063870601800216","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:26:52Z","timestamp":1305217612000},"page":"218-221","source":"Crossref","is-referenced-by-count":33,"title":["Identification of Pathogenic\n                    <i>Leptospira<\/i>\n                    Strains in Tissues of a Premature Foal by Use of Polymerase Chain Reaction Analysis"],"prefix":"10.1177","volume":"18","author":[{"given":"Albertine","family":"L\u00e9on","sequence":"first","affiliation":[{"name":"Laboratoire D\u00e9partemental Frank Duncombe, 1 route de Rosel, 14053 Caen cedex, France"},{"name":"Laboratoire de Microbiologie CHU C\u00f4te de Nacre (EA 2128), Universit\u00e9 de Caen, 14033 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Pronost","sequence":"additional","affiliation":[{"name":"Laboratoire D\u00e9partemental Frank Duncombe, 1 route de Rosel, 14053 Caen cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie","family":"Tapprest","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etudes et de Recherches en Pathologie Equine, AFSSA site de Dozul\u00e9, 14430 Goustranville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Foucher","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etudes et de Recherches en Pathologie Equine, AFSSA site de Dozul\u00e9, 14430 Goustranville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Adiag\u00e8ne, 38 rue de Paris, 22000 Saint Brieuc, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Andr\u00e9-Fontaine","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie M\u00e9dicale et Mol\u00e9culaire des Leptospires, Ecole Nationale V\u00e9t\u00e9rinaire de Nantes, Atlanpole la Chantrerie, BP 40706, 44307 Nantes Cedex 3, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Laugier","sequence":"additional","affiliation":[{"name":"Laboratoire d'Etudes et de Recherches en Pathologie Equine, AFSSA site de Dozul\u00e9, 14430 Goustranville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Fortier","sequence":"additional","affiliation":[{"name":"Laboratoire D\u00e9partemental Frank Duncombe, 1 route de Rosel, 14053 Caen cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie CHU C\u00f4te de Nacre (EA 2128), Universit\u00e9 de Caen, 14033 Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,3,1]]},"reference":[{"key":"bibr1-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.8.1894-1898.1994"},{"key":"bibr2-104063870601800216","unstructured":"Belak S, Ballagi-Pordany A, Timoney PJ, 1994, Evaluation of a nested PCR assay for the detection of equine arteritis virus. 7th Int Conf Equine Inf Dis (Tokyo), pp. 33\u201339."},{"key":"bibr3-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.01.007"},{"key":"bibr4-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1136\/vr.136.18.463"},{"key":"bibr5-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1177\/104063879500700114"},{"key":"bibr6-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1177\/104063879100300208"},{"key":"bibr7-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1983.tb01811.x"},{"key":"bibr8-104063870601800216","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1993.203.08.1170"},{"key":"bibr9-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-139-8-1691"},{"key":"bibr10-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1177\/104063878900100401"},{"key":"bibr11-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.4.1035-1039.1994"},{"key":"bibr12-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(93)90128-T"},{"key":"bibr13-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"bibr14-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45860-0"},{"key":"bibr15-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.9.2219-2224.1992"},{"key":"bibr16-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1997.tb10262.x"},{"key":"bibr17-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651997000400004"},{"key":"bibr18-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700114"},{"key":"bibr19-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2503"},{"key":"bibr20-104063870601800216","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00066-9"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870601800216","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870601800216","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:11:00Z","timestamp":1777680660000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870601800216"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1177\/104063870601800216"],"URL":"https:\/\/doi.org\/10.1177\/104063870601800216","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,3]]}}}