{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:16:50Z","timestamp":1791915410277,"version":"4.3.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:p>Erysipelothrix rhusiopathiae is the causative agent of swine erysipelas, and it causes great economic losses in Japan and worldwide. In meat inspection, it is very important to distinguish E. rhusiopathiae from other bacteria showing similar clinical signs of disease or similar bacterial characteristics. To distinguish E. rhusiopathiae from Erysipelothrix tonsillarum, 2 polymerase chain reaction (PCR) systems were combined. The primer sets ERY-1F and ERY-2R were designed to amplify 2,210 base pairs (bp) of nucleotide sequence specific for E. rhusiopathiae chromosomal DNA, and the primer sets MO101 and ERS-1R were designed to amplify 719 bp of nucleotide sequence including a highly conserved region of genus Erysipelothrix 16S rRNA. Two fragments were amplified when E. rhusiopathiae was used as the PCR template using the primer sets, whereas a single fragment was amplified when E. tonsillarum was used as the template.<\/jats:p>","DOI":"10.1177\/104063870601800411","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:26:52Z","timestamp":1305217612000},"page":"384-387","source":"Crossref","is-referenced-by-count":30,"title":["A Multiplex Polymerase Chain Reaction for Discriminating\n                    <i>Erysipelothrix Rhusiopathiae<\/i>\n                    from\n                    <i>Erysipelothrix Tonsillarum<\/i>"],"prefix":"10.1177","volume":"18","author":[{"given":"Yoshinao","family":"Yamazaki","sequence":"first","affiliation":[{"name":"Ibaraki Western District Meat Inspection Office 584 Ichinobe, Chikusei, Ibaraki 308-0027, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,7,1]]},"reference":[{"key":"bibr1-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-382"},{"key":"bibr2-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-9-789"},{"key":"bibr3-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.2.371-376.1994"},{"key":"bibr4-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.5.2066-2070.2000"},{"key":"bibr5-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-23-2-184"},{"key":"bibr6-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(98)00152-4"},{"key":"bibr7-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.6.1526-1531.1994"},{"key":"bibr8-104063870601800411","first-page":"150","volume-title":"Animal Hygiene Weekly","author":"Ministry of Agriculture, Forestry &amp; Fisheries of Japan","year":"2005"},{"key":"bibr9-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.12.4332-4336.2000"},{"key":"bibr10-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.4.354"},{"key":"bibr11-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.86-89.1998"},{"key":"bibr12-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-469"},{"key":"bibr13-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.25.3.536-539.1987"},{"key":"bibr14-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4093-4098.1999"},{"key":"bibr15-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.01.022"},{"key":"bibr16-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.12.6455-6467.1989"},{"key":"bibr17-104063870601800411","doi-asserted-by":"publisher","DOI":"10.1128\/JB.82.4.595-599.1961"},{"key":"bibr18-104063870601800411","doi-asserted-by":"crossref","first-page":"944","DOI":"10.2460\/javma.1984.184.08.944","volume":"184","author":"Wood RL","year":"1984","journal-title":"J Am Vet Assoc"},{"key":"bibr19-104063870601800411","first-page":"419","volume-title":"Diseases of swine","author":"Wood RL","year":"2000","edition":"8"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870601800411","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063870601800411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:11:10Z","timestamp":1777680670000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063870601800411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,7]]}},"alternative-id":["10.1177\/104063870601800411"],"URL":"https:\/\/doi.org\/10.1177\/104063870601800411","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7]]}}}