{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:34:05Z","timestamp":1777930445641,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>In the dog, early-stage epitheliotropic T-cell lymphoma (ETCL) can clinically and histologically mimic a large range of inflammatory dermatoses and often progresses rapidly to a more aggressive tumor stage. Early diagnosis of ETCL is essential to proceed with a specific oncologic therapy that is favorable for the prognosis. In the present study, an improved method for the detection of T-cell receptor gamma (TCR\u03b3) rearrangement was developed by designing a new set of consensus primers to amplify the different forms of rearranged canine TCR\u03b3 gene sequences by polymerase chain reaction. The amplicons were analyzed by conventional polyacrylamide gel electrophoresis, which requires minimal specific equipment and may be performed in almost every pathology laboratory at low costs. The method proved to be highly specific and sensitive to detect early ETCL in formalin-fixed, paraffin-embedded biopsy specimens, providing an efficient tool for veterinary pathologists to distinguish early neoplastic from reactive cutaneous T-cell infiltrates (tumor-specific marker) or to discriminate T-cell lymphoma from B-cell lymphomas or nonlymphoid neoplasms (T-cell lineage marker). By direct sequencing analysis of amplified TCR\u03b3 gene sequences, ETCL was found to rearrange exclusively the joining (J) 4 region, which suggests specific biology for primary cutaneous T-cell lymphomas. Also, a novel (seventh) functional J region in the TCR\u03b3 gene, localized approximately 2.3 kb upstream of J5, was identified.<\/jats:p>","DOI":"10.1177\/104063871002200104","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:29:05Z","timestamp":1305217745000},"page":"20-29","source":"Crossref","is-referenced-by-count":19,"title":["Improved Polymerase Chain Reaction\u2013Based Method to Detect Early-Stage Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides) in Formalin-Fixed, Paraffin-Embedded Skin Biopsy Specimens of the Dog"],"prefix":"10.1177","volume":"22","author":[{"given":"Pascal","family":"Chaubert","sequence":"first","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."},{"name":"Institute of Human Pathology, Faculty of Medicine and Biology, University of Lausanne, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey S. Baur","family":"Chaubert","sequence":"additional","affiliation":[{"name":"Institute of Human Pathology, Faculty of Medicine and Biology, University of Lausanne, Lausanne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Sattler","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Forster","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rie","family":"Bornand","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Suter","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Welle","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse-Faculty, University of Berne, Berne, Switzerland."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,1,1]]},"reference":[{"key":"bibr1-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-200002000-00009"},{"key":"bibr2-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-199506000-00006"},{"key":"bibr3-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.9.3033.409k39_3033_3043"},{"key":"bibr4-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1354\/vp.40-1-32"},{"key":"bibr5-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00047-1"},{"key":"bibr6-104063871002200104","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1002\/9780470752487","volume-title":"Skin diseases of the dog and cat: clinical and histopathologic diagnosis","author":"Gross TL","year":"2005","edition":"2"},{"key":"bibr7-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.04.050"},{"key":"bibr8-104063871002200104","first-page":"421","volume":"144","author":"Moore PF","year":"1994","journal-title":"Am J Pathol"},{"key":"bibr9-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30181-5"},{"key":"bibr10-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2007.10.028"},{"key":"bibr11-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711740402"},{"key":"bibr12-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210824"},{"key":"bibr13-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2427(95)05519-3"},{"key":"bibr14-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2005.02.020"},{"key":"bibr15-104063871002200104","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.11.005"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063871002200104","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/104063871002200104","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/104063871002200104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:13:47Z","timestamp":1777680827000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/104063871002200104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1177\/104063871002200104"],"URL":"https:\/\/doi.org\/10.1177\/104063871002200104","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}