{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:35:06Z","timestamp":1777930506932,"version":"3.51.4"},"reference-count":18,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p>Mycoplasma bovis is an important pathogen of cattle, causing mastitis, pneumonia, conjunctivitis, otitis, and arthritis. Currently there are only a few reports of sensitivity levels for M. bovis isolates from the United States. Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory between December 2007 and December 2008 (n = 192) were tested for antimicrobial susceptibility to enrofloxacin, erythromycin, florfenicol, spectinomycin, ceftiofur, tetracycline, and oxytetracycline using a broth microdilution method. The most effective antimicrobials against M. bovis determined by using the broth microdilution method were florfenicol, enrofloxacin, and tetracycline with minimum inhibitory concentration (MIC) ranges of 2\u201332 \u00b5g\/ml, 0.1\u20133.2 \u00b5g\/ml, and 0.05 to &gt;12.8 \u00b5g\/ml, respectively. Spectinomycin, oxytetracycline, and tetracycline showed a wide-ranging level of efficacy in isolate inhibition with broth microdilution with MIC ranges of 4 to &gt;256 \u00b5g\/ml, 0.05 to &gt;12.8 \u00b5g\/ml, and 0.05 to &gt;12.8 \u00b5g\/ml, respectively. A significant difference in the susceptibility levels between quarter milk and lung isolates was found for spectinomycin. When MIC values of a subset of the M. bovis isolates (n = 12) were tested using a flow cytometric technique, the MIC ranges of enrofloxacin, spectinomycin, ceftiofur, erythromycin, tetracycline, oxytetracycline, and florfenicol ranges were 0.1\u20130.4 \u00b5g\/ml, 4 to &gt;256 \u00b5g\/ml, &gt;125 \u00b5g\/ml, &gt;3.2 \u00b5g\/ml, &lt;0.025 to &gt;6.4 \u00b5g\/ml, 0.8 to &gt;12.8 \u00b5g\/ml, and &lt;2\u20134 \u00b5g\/ml, respectively. Flow cytometry offers potential in clinical applications due to high-throughput capability, quick turnaround time, and the objective nature of interpreting results.<\/jats:p>","DOI":"10.1177\/1040638711404155","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T16:29:05Z","timestamp":1305217745000},"page":"547-551","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["In vitro antimicrobial inhibition of\n                    <i>Mycoplasma bovis<\/i>\n                    isolates submitted to the Pennsylvania Animal Diagnostic Laboratory using flow cytometry and a broth microdilution method"],"prefix":"10.1177","volume":"23","author":[{"given":"Marty K.","family":"Soehnlen","sequence":"first","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Elaine","family":"Kunze","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Eranda","family":"Karunathilake","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittnee M.","family":"Henwood","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhashinie","family":"Kariyawasam","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Wolfgang","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhushan M.","family":"Jayarao","sequence":"additional","affiliation":[{"name":"Department of Veterinary & Biomedical Sciences (Soehnlen, Karunathilake, Henwood, Kariyawasam, Wolfgang, Jayarao) and Huck Institutes of Life Sciences (Kunze), The Pennsylvania State University, and The Pennsylvania Animal Diagnostics Laboratory (Kariyawasam, Jayarao), University Park, PA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,5,1]]},"reference":[{"key":"bibr1-1040638711404155","first-page":"1132","volume":"102","author":"Assuncao P","year":"2006","journal-title":"J Appl Microbiol"},{"key":"bibr2-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01582-05"},{"key":"bibr3-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20331"},{"key":"bibr4-1040638711404155","doi-asserted-by":"publisher","DOI":"10.2741\/1812"},{"key":"bibr5-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1136\/vr.146.26.745"},{"key":"bibr6-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.10.006"},{"key":"bibr7-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.01.028"},{"key":"bibr8-1040638711404155","first-page":"582","volume":"52","author":"Hale H","year":"1962","journal-title":"Cornell Vet"},{"key":"bibr9-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2000100"},{"key":"bibr10-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)31146-9"},{"key":"bibr11-1040638711404155","volume-title":"NCCLS document M31-A2","author":"National Committee for Clinical Laboratory Standards (NCCLS)","year":"2002"},{"key":"bibr12-1040638711404155","volume-title":"NCCLS document M100-S16","author":"National Committee for Clinical Laboratory Standards (NCCLS)","year":"2006"},{"key":"bibr13-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(02)00155-8"},{"key":"bibr14-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2008.08.004"},{"key":"bibr15-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700505"},{"key":"bibr16-1040638711404155","doi-asserted-by":"publisher","DOI":"10.2307\/1591770"},{"key":"bibr17-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1016\/S1090-0233(03)00186-2"},{"key":"bibr18-1040638711404155","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.10.2571"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638711404155","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638711404155","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638711404155","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:15:23Z","timestamp":1777680923000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638711404155"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1177\/1040638711404155"],"URL":"https:\/\/doi.org\/10.1177\/1040638711404155","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5]]}}}