{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:35:05Z","timestamp":1777930505782,"version":"3.51.4"},"reference-count":16,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,6,15]],"date-time":"2011-06-15T00:00:00Z","timestamp":1308096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>A 15-year-old Belgian gelding was referred for fever, depression, and respiratory distress. Lung biopsy revealed interstitial fibrosis consistent with chronic interstitial pneumonia. Equid herpesvirus 5 (EHV-5) DNA was detected by polymerase chain reaction (PCR) in bronchoalveolar lavage and biopsy specimens. A presumptive diagnosis of equine multinodular pulmonary fibrosis (EMPF) was made, and the horse was administered a systemic treatment with corticosteroids and antiviral drugs. Despite initial clinical improvement, 4 weeks later, the condition of the horse rapidly deteriorated, and the animal was euthanized. Postmortem examination confirmed the presumptive diagnosis of EMPF. The EHV-5 DNA load in different tissues was estimated using a quantitative real-time PCR. Lung had a remarkable viral load, higher than in other organs, especially within the pulmonary fibrotic nodules, and a linkage between high viral burden and the most severely affected tissues was observed. The results suggest that the quantitative real-time PCR is a useful tool to quantify the EHV-5 load in different organs and to understand the relationship between EHV-5 and EMPF. The bronchoalveolar lavage was determined to be a good clinical sample to estimate the EHV-5 load in lung.<\/jats:p>","DOI":"10.1177\/1040638711407890","type":"journal-article","created":{"date-parts":[[2011,6,16]],"date-time":"2011-06-16T14:22:36Z","timestamp":1308234156000},"page":"802-806","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Quantification of\n                    <i>Equid herpesvirus 5<\/i>\n                    DNA in clinical and necropsy specimens collected from a horse with equine multinodular pulmonary fibrosis"],"prefix":"10.1177","volume":"23","author":[{"given":"Maria Luisa","family":"Marenzoni","sequence":"first","affiliation":[{"name":"Sports Horse Research Centre, Department of Experimental Science and Applied Biotechnology, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy (Marenzoni, Passamonti, Lepri, Cercone, Capomaccio, Cappelli, Felicetti, Coppola, Coletti)"},{"name":"Virology and Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Li\u00e8ge, Li\u00e8ge, Belgium (Thiry)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Passamonti","sequence":"additional","affiliation":[{"name":"Sports Horse Research Centre, Department of Experimental Science and Applied Biotechnology, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy (Marenzoni, Passamonti, Lepri, Cercone, Capomaccio, Cappelli, Felicetti, Coppola, Coletti)"},{"name":"Virology and Viral Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Li\u00e8ge, Li\u00e8ge, Belgium (Thiry)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvio","family":"Lepri","sequence":"additional","affiliation":[{"name":"Sports Horse Research Centre, Department of Experimental Science and Applied Biotechnology, Faculty of 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