{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:35:09Z","timestamp":1777930509759,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T00:00:00Z","timestamp":1307404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>\n                    A SYTO9 real-time polymerase chain reaction assay for detection of pathogenic Leptospira spp. based on amplification of DNA gyrase subunit B ( gyrB) gene has been optimized and evaluated for sensitivity and specificity on kidney and urine samples of New Zealand farmed deer. The detection limit was 10\n                    <jats:sup>3<\/jats:sup>\n                    cells\/ml (2\u201310 copies\/reaction). Comparison of the assay on deer kidneys (n = 268) with culture as the gold standard revealed a sensitivity and specificity of 85% and 99.2%, respectively. For deer urine (n = 113), the assay was compared with known inoculated samples and revealed a sensitivity and specificity of 96.7% and 100%, respectively. The assay was applied for quantifying pathogenic leptospires shed naturally in deer urine and revealed a detectable concentration of 3.7 \u00d7 10\n                    <jats:sup>3<\/jats:sup>\n                    to 1.7 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    cells\/ml. To assess the assay\u2019s capability for identifying pathogenic Leptospira spp., 14 field isolates of L. borgpetersenii serovar Hardjo-bovis and L. interrogans serovar Pomona were amplified for polymerase chain reaction (PCR) product, purified, and sequenced. When compared with the National Center for Biotechnology Information database, sequence data matched with L. borgpetersenii serovar Hardjo-bovis in 13 samples and L. interrogans serovar Pomona in 1 sample, which was consistent with the microscopic agglutination test (MAT). Sequence analysis of purified PCR product amplified directly from kidney and urine samples also yielded serovar-comparable MAT results. Results suggest that the assay is rapid, sensitive, and specific for detection of pathogenic leptospires in deer clinical samples. The developed assay can also be used for estimating the concentration of leptospires and identifying Leptospira spp. in combination with DNA sequencing.\n                  <\/jats:p>","DOI":"10.1177\/1040638711407892","type":"journal-article","created":{"date-parts":[[2011,6,7]],"date-time":"2011-06-07T21:58:12Z","timestamp":1307483892000},"page":"743-752","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of a SYTO9 real-time polymerase chain reaction assay to detect and identify pathogenic\n                    <i>Leptospira<\/i>\n                    species in kidney tissue and urine of New Zealand farmed deer"],"prefix":"10.1177","volume":"23","author":[{"given":"Supatsak","family":"Subharat","sequence":"first","affiliation":[{"name":"Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter R.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cord","family":"Heuer","sequence":"additional","affiliation":[{"name":"Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie M.","family":"Collins-Emerson","sequence":"additional","affiliation":[{"name":"Institute of Veterinary, Animal and Biomedical Sciences Massey University, Palmerston North, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,6,7]]},"reference":[{"key":"bibr1-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-5-28"},{"key":"bibr2-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2007.36750"},{"key":"bibr3-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2010.68863"},{"key":"bibr4-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.8.1894-1898.1994"},{"key":"bibr5-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"bibr6-1040638711407892","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.1982.34873"},{"key":"bibr7-1040638711407892","first-page":"278","volume":"118","author":"Bomfim MRQ","year":"2006","journal-title":"in urine samples of cattle with clinical suspicion of leptospirosis. 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