{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T10:31:48Z","timestamp":1791973908204,"version":"4.4.0"},"reference-count":43,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2011,12,6]],"date-time":"2011-12-06T00:00:00Z","timestamp":1323129600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>Cutaneous mast cell tumors (MCTs) are among the most common neoplasms in dogs and show a highly variable biologic behavior. Histological grading, cell proliferation markers, and KIT immunohistochemistry are typically used to predict post-surgical prognosis. In the present study, c-KIT messenger RNA (mRNA) expression was measured in canine MCTs and its relationship with tumor grade, immunohistochemical staining pattern, post-surgical prognosis, and mutations was investigated. A significant increase of c-KIT mRNA was observed in MCTs versus healthy skin and surgical margins. Mutations were observed in 8.3% of cases. The KIT staining pattern was investigated for both grading systems. In particular, staining pattern III was associated with grade II (G2) and G3 MCTs, while staining patterns I and II were associated with G1 and G2 MCTs. Considering the 2-tier histological grading, the high grade was mainly associated with pattern III (71%) while the low grade was associated with patterns II (70%) and I (28%). A weak association between the KIT staining pattern and outcome was also observed. The results obtained suggest that c-KIT mRNA is overexpressed in canine MCT, although the fold variations were not associated with the protein localization or complementary DNA mutations. These observations suggested that the 3 events were independent. The histological grading and the KIT staining pattern have prognostic value as previously published. Staining pattern I could be especially helpful in predicting a good prognosis of G2 MCTs. Sequence mutations were not necessarily suggestive of a worse prognosis, but might be useful in choosing a chemotherapy protocol.<\/jats:p>","DOI":"10.1177\/1040638711425945","type":"journal-article","created":{"date-parts":[[2011,12,7]],"date-time":"2011-12-07T00:40:07Z","timestamp":1323218407000},"page":"116-126","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":42,"title":["c-KIT messenger RNA and protein expression and mutations in canine cutaneous mast cell tumors"],"prefix":"10.1177","volume":"24","author":[{"given":"Mery","family":"Giantin","sequence":"first","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Vascellari","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela Maria","family":"Morello","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia","family":"Capello","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Vercelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Granato","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa Maria","family":"Lopparelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Nassuato","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Carminato","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Martano","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Mutinelli","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Dacasto","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanit\u00e0 Pubblica, Patologia Comparata ed Igiene Veterinaria, Universit\u00e0 di Padova, viale dell\u2019Universit\u00e0 16, Agripolis Legnaro, Padova, Italy (Giantin, Lopparelli, Dacasto)"},{"name":"Istituto Zooprofilattico Sperimentale delle Venezie, viale dell\u2019Universit\u00e0 10, Legnaro, Padova, Italy (Vascellari, Capello, Granato, Nassuato, Carminato, Mutinelli)"},{"name":"Dipartimento di Patologia Animale, Universit\u00e0 di Torino, via Leonardo da Vinci 44, Grugliasco, Turin, Italy (Morello, Martano)"},{"name":"Ambulatorio Veterinario Associato, Torino, Italy (Vercelli)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,12,6]]},"reference":[{"key":"bibr1-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.2.135"},{"key":"bibr2-1040638711425945","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2002.63.1718"},{"key":"bibr3-1040638711425945","doi-asserted-by":"publisher","DOI":"10.2460\/javma.228.2.210"},{"key":"bibr4-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-3-19"},{"key":"bibr5-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1002\/9780470376928.ch2"},{"key":"bibr6-1040638711425945","first-page":"28","volume-title":"WHO classification of tumours of domestic animals","author":"Hendrick MJ","year":"1998"},{"key":"bibr7-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1159\/000101711"},{"key":"bibr8-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/104063870401600201"},{"key":"bibr9-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810386469"},{"key":"bibr10-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1354\/vp.41-4-371"},{"key":"bibr11-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2005.00726.x"},{"key":"bibr12-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2009.00185.x"},{"key":"bibr13-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.60.1335"},{"key":"bibr14-1040638711425945","first-page":"226","volume":"40","author":"Lee S","year":"2007","journal-title":"J Biochem Mol Biol"},{"key":"bibr15-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-08-0067"},{"key":"bibr16-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(99)00078-3"},{"key":"bibr17-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"bibr18-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2009.02.002"},{"key":"bibr19-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(98)00075-7"},{"key":"bibr20-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(96)80074-0"},{"key":"bibr21-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00003-2"},{"key":"bibr22-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/002215540405200515"},{"key":"bibr23-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.06.014"},{"key":"bibr24-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.69.1117"},{"key":"bibr25-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1080\/08923970801949265"},{"key":"bibr26-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/030098588402100503"},{"key":"bibr27-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2003.10.003"},{"key":"bibr28-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-59524-0_3"},{"key":"bibr29-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700416"},{"key":"bibr30-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44-3-335"},{"key":"bibr31-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2006.tb02835.x"},{"key":"bibr32-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/030098589403100602"},{"key":"bibr33-1040638711425945","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.224.236"},{"key":"bibr34-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/B978-072160558-6.50022-8"},{"key":"bibr35-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810387446"},{"key":"bibr36-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1177\/104063870601800404"},{"key":"bibr37-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-6-85"},{"key":"bibr38-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1593\/neo.05622"},{"key":"bibr39-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44-3-298"},{"key":"bibr40-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-4-32"},{"key":"bibr41-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00694.x"},{"key":"bibr42-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1354\/vp.39-5-529"},{"key":"bibr43-1040638711425945","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(01)02217-1"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638711425945","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638711425945","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638711425945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:16:03Z","timestamp":1777680963000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638711425945"}},"subtitle":["correlations with post-surgical prognosis"],"short-title":[],"issued":{"date-parts":[[2011,12,6]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1177\/1040638711425945"],"URL":"https:\/\/doi.org\/10.1177\/1040638711425945","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,6]]}}}