{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:33:09Z","timestamp":1790148789387,"version":"4.0.1"},"reference-count":18,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>The objective of the current study was to isolate and identify phenotypes of Staphylococcus spp. strains derived from buffalo ( Bubalus bubalis) milk. A total of 548 milk samples from 137 buffalo were cultured in Columbia agar enriched with 5% defibrinated sheep blood. Determination of the capacity of Staphylococcus aureus to produce enterotoxins A\u2013D and toxic shock syndrome toxin-1 (TSST-1) was achieved by reverse passive latex agglutination (RPLA). Antimicrobial sensitivity of S. aureus strains was evaluated using the disk diffusion technique, and \u03b2-lactamase detection was achieved using the chromogenic test with paper discs impregnated with nitrocefin. From all the mammary quarters examined, 36 (10.8%) were positive for Staphylococcus spp., 83.3% were coagulase-negative staphylococcus (CNS), 11.1% were coagulase-positive staphylococcus (CPS), and 5.6% were of CPS+CNS positive. All isolates of S. aureus produced at least 1 toxin and 5 out of 6 isolates (83.0%) produced \u03b2-lactamase. One hundred percent of S. aureus isolates were sensitive to methicillin and amoxicillin + clavulanic acid, and resistant to ampicillin, penicillin, and oxacillin. Analysis of the results obtained in the current study highlight the epidemiologic importance of buffalo milk regarding the production of enterotoxins and TSST-1 and the potential risk to public health.<\/jats:p>","DOI":"10.1177\/1040638711428946","type":"journal-article","created":{"date-parts":[[2011,11,15]],"date-time":"2011-11-15T04:04:53Z","timestamp":1321329893000},"page":"1208-1211","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Phenotype characterization of\n                    <i>Staphylococcus<\/i>\n                    species strains isolated from buffalo (\n                    <i>Bubalus bubalis<\/i>\n                    ) milk"],"prefix":"10.1177","volume":"23","author":[{"given":"Andrea A. F.","family":"Oliveira","sequence":"first","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Jos\u00e9 W.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinaldo A.","family":"Mota","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L. R. S.","family":"Cunha","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos A. M.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noeme S.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil (Oliveira, Mota)"},{"name":"Federal Rural University of Pernambuco, Academic Unit of Garanhuns, Garanhuns, Pernambuco, Brazil (Pinheiro Jr)"},{"name":"Department of Microbiology and Immunology (Cunha, Lopes), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"},{"name":"Faculty of Veterinary and Animal Husbandry (Rocha), S\u00e3o Paulo State University J\u00falio de Mesquita Filho, Botucatu, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,11,14]]},"reference":[{"key":"bibr1-1040638711428946","first-page":"205","volume":"31","author":"Almeida RA","year":"2001","journal-title":"Staphylococcus"},{"key":"bibr2-1040638711428946","doi-asserted-by":"publisher","DOI":"10.3923\/ijds.2009.117.122"},{"key":"bibr3-1040638711428946","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00377-9"},{"key":"bibr4-1040638711428946","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352000000100002"},{"key":"bibr5-1040638711428946","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-09352007000100039"},{"key":"bibr6-1040638711428946","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.69.1241"},{"key":"bibr7-1040638711428946","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-007-9057-6"},{"key":"bibr8-1040638711428946","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72165-8"},{"key":"bibr9-1040638711428946","first-page":"291","volume-title":"Indian Vet J","volume":"75","author":"Naiknaware HS","year":"1998"},{"key":"bibr10-1040638711428946","unstructured":"National Committee for Clinical Laboratory Standard (NCCLS): 2000, Performance standards for antimicrobial susceptibility testing\u2014twelfth informational supplement. 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