{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:35:50Z","timestamp":1777930550098,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T00:00:00Z","timestamp":1330473600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs for the presence of Haemophilus parasuis and predict the virulence of the strains present. Nasal cavity swabs were taken from 30 live, healthy, 1- to 8-week-old pigs on a weekly cycle from a commercial Thai nursery pig herd. A total of 27 of these pigs (90%) tested positive for H. parasuis as early as week 1 of age. None of the H. parasuis\u2013positive samples from healthy pigs was positive for the hhdBA genes. At the same pig nursery, swab samples from nasal cavity, tonsil, trachea, and lung, and exudate samples from pleural\/peritoneal cavity were taken from 30 dead pigs displaying typical pathological lesions consistent with Gl\u00e4sser disease. Twenty-two of 140 samples (15.7%) taken from 30 diseased pigs yielded a positive result for H. parasuis. Samples from the exudate (27%) yielded the most positive results, followed by lung, tracheal swab, tonsil, and nasal swab, respectively. Out of 22 positive samples, 12 samples (54.5%) harbored hhdA and\/or hhdB genes. Detection rates of hhdA were higher than hhdB. None of the H. parasuis\u2013positive samples taken from nasal cavity of diseased pigs tested positive for hhdBA genes. More work is required to determine if the detection of hhdBA genes is useful for identifying the virulence potential of H. parasuis field isolates.<\/jats:p>","DOI":"10.1177\/1040638711435805","type":"journal-article","created":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T20:00:02Z","timestamp":1330545602000},"page":"339-343","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of a putative hemolysin operon,\n                    <i>hhdBA<\/i>\n                    , of\n                    <i>Haemophilus parasuis<\/i>\n                    from pigs with Gl\u00e4sser disease"],"prefix":"10.1177","volume":"24","author":[{"given":"Pornchalit","family":"Assavacheep","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand (P Assavacheep)"},{"name":"Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand (A Assavacheep)"},{"name":"The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Dutton Park, Queensland, Australia (Turni)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anongnart","family":"Assavacheep","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand (P Assavacheep)"},{"name":"Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University, Pathumwan, Bangkok, Thailand (A Assavacheep)"},{"name":"The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Dutton Park, Queensland, Australia (Turni)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conny","family":"Turni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Veterinary 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