{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:30:36Z","timestamp":1790033436468,"version":"4.0.1"},"reference-count":24,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>The concomitant infections of Canine distemper virus (CDV), Canine adenovirus A types 1 (CAdV-1) and 2 (CAdV-2), Canine parvovirus type 2 (CPV-2), and Toxoplasma gondii are described in a 43-day-old mixed-breed puppy. Clinically, there were convulsions and blindness with spontaneous death; 14 siblings of this puppy, born to a 10-month-old dam, which was seropositive (titer: 1,024) for T. gondii, also died. Necropsy revealed unilateral corneal edema (blue eye), depletion of intestinal lymphoid tissue, non-collapsible lungs, congestion of meningeal vessels, and a pale area in the myocardium. Histopathology demonstrated necrotizing myocarditis associated with intralesional apicomplexan protozoa; necrotizing and chronic hepatitis associated with rare intranuclear inclusion bodies within hepatocytes; necrotizing bronchitis and bronchiolitis; interstitial pneumonia associated with eosinophilic intracytoplasmic inclusion bodies within epithelial cells; atrophy and fusion of intestinal villi with cryptal necrosis; and white matter demyelination of the cerebrum and cerebellum associated with intranuclear inclusion bodies within astrocytes. Polymerase chain reaction (PCR) amplified the partial fragments (bp) of the CDV N gene (290 bp), CPV-2c VP2 capsid protein gene (583 bp), and CAdV-1 (508 bp) and CAdV-2 (1,030 bp) E gene from urine and tissue samples. The PCR assays demonstrated that the apicomplexan protozoa observed within several organs contained DNA specific for T. gondii; genotyping revealed T. gondii type III. The findings support the characterization of concomitant infections of CDV, CAdV-1, CAdV-2, CPV-2, and T. gondii in this puppy. Further, seroreactivity to T. gondii of the dam in association with the systemic disease observed in the puppy described herein is suggestive of congenital toxoplasmosis.<\/jats:p>","DOI":"10.1177\/1040638712471344","type":"journal-article","created":{"date-parts":[[2013,1,5]],"date-time":"2013-01-05T03:04:51Z","timestamp":1357355091000},"page":"129-135","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":65,"title":["Concomitant canine distemper, infectious canine hepatitis, canine parvoviral enteritis, canine infectious tracheobronchitis, and toxoplasmosis in a puppy"],"prefix":"10.1177","volume":"25","author":[{"given":"Selwyn Arlington","family":"Headley","sequence":"first","affiliation":[{"name":"Laboratories of Animal Virology (Headley, AA Alfieri, Fritzen, Silva, Bodnar, AF Alfieri), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paran\u00e1, Brazil"},{"name":"Laboratories of Parasitology (Garcia), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paran\u00e1, Brazil"},{"name":"Department of Pathobiology, Institute of Pathology and Forensic Veterinary Medicine, University of Veterinary Medicine, Vienna, Austria (Weissenb\u00f6ck)"},{"name":"Laboratory of Veterinary Pathology, School of Veterinary Medicine, Universidade Norte do Paran\u00e1, Arapongas, Paran\u00e1, Brazil (Okano)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amauri Alcindo","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Laboratories of Animal Virology (Headley, AA Alfieri, Fritzen, Silva, Bodnar, AF Alfieri), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paran\u00e1, Brazil"},{"name":"Laboratories of Parasitology (Garcia), Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paran\u00e1, Brazil"},{"name":"Department of Pathobiology, Institute of Pathology and 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