{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:36:11Z","timestamp":1777930571700,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T00:00:00Z","timestamp":1365465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>\n                    A species-independent indirect enzyme-linked immunosorbent assay (iELISA) based on chimeric protein A\/G was established for the detection of anti-\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    antibodies in Arctic wildlife species and compared to previously established brucellosis serological tests for hooded seals (\n                    <jats:italic toggle=\"yes\">Cystophora cristata<\/jats:italic>\n                    ), minke whales (\n                    <jats:italic toggle=\"yes\">Balaenoptera acutorostrata<\/jats:italic>\n                    ), sei whales (\n                    <jats:italic toggle=\"yes\">Balaenoptera borealis<\/jats:italic>\n                    ), fin whales (\n                    <jats:italic toggle=\"yes\">Balaenoptera physalus<\/jats:italic>\n                    ), and polar bears (\n                    <jats:italic toggle=\"yes\">Ursus maritimus<\/jats:italic>\n                    ), as well as bacteriology results for reindeer and caribou (\n                    <jats:italic toggle=\"yes\">Rangifer tarandus<\/jats:italic>\n                    sp.). The protein A\/G iELISA results were consistent with the other serological tests with Cohen kappa values between 0.47 and 0.92, and the protein A\/G iELISA can thus offer a technically simple method for these species yielding results consistent with established brucellosis serological tests. Receiver operator characteristics analysis proved that the reindeer and caribou protein A\/G iELISA results were consistent with the bacteriological gold standard with an area under the curve of 0.99, and the protein A\/G iELISA was thus validated as a sensitive and specific serological method for the detection of anti-\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    antibodies in reindeer and caribou. The binding of the antibodies from the respective species to protein A and G were also evaluated in the iELISA. The antibodies from hooded seals and polar bears reacted stronger to protein A than to G. The sei whale, fin whale, reindeer, and caribou antibodies reacted stronger to protein G than to A. The minke whale antibodies reacted to both protein A and G. There was a strong correlation (\n                    <jats:italic toggle=\"yes\">r<\/jats:italic>\n                    <jats:sub>s<\/jats:sub>\n                    = 0.88\u20130.98) between the optical density results obtained with the iELISA with protein A\/G and protein A or G, showing that protein A\/G is as well suited as protein A or G for the detection of anti-\n                    <jats:italic toggle=\"yes\">Brucella<\/jats:italic>\n                    antibodies in these species with the iELISA.\n                  <\/jats:p>","DOI":"10.1177\/1040638713485073","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T00:36:59Z","timestamp":1365554219000},"page":"369-375","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":36,"title":["A protein A\/G indirect enzyme-linked immunosorbent assay for the detection of anti-\n                    <i>Brucella<\/i>\n                    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K.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Section for Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Troms\u00f8, Norway (Nymo, Godfroid, \u00c5sbakk, Larsen, das Neves, Tryland)"},{"name":"Bioforsk\u2013Norwegian Institute of Agricultural and Environmental Research, Troms\u00f8, Norway (R\u00f8dven)"},{"name":"Members of the Fram Centre\u2013High North Research Centre for Climate and the Environment, Troms\u00f8, Norway (Nymo, Godfroid, \u00c5sbakk, Larsen, das Neves, R\u00f8dven, Tryland)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos G.","family":"das Neves","sequence":"additional","affiliation":[{"name":"Section for Arctic Veterinary Medicine, The Norwegian School of Veterinary Science, Troms\u00f8, Norway (Nymo, Godfroid, \u00c5sbakk, Larsen, das Neves, Tryland)"},{"name":"Bioforsk\u2013Norwegian Institute of Agricultural and Environmental Research, Troms\u00f8, Norway (R\u00f8dven)"},{"name":"Members of 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