{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:36:18Z","timestamp":1777930578373,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T00:00:00Z","timestamp":1375056000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>\n                    Paragonimus heterotremus is a medically important lung fluke that causes human and animal paragonimiasis in Southeast Asia, including Thailand. In the current study, a real-time fluorescence resonance energy transfer polymerase chain reaction (real-time FRET PCR) with melting curve analysis was developed and evaluated to detect P. heterotremus eggs in the feces of experimentally infected cats. The detection limit of this method for the P. heterotremus DNA sequence was 3 \u00d7 10\n                    <jats:sup>2<\/jats:sup>\n                    copies of the positive control plasmid and 10\n                    <jats:sup>\u22123<\/jats:sup>\n                    ng of P. heterotremus genomic DNA. The assay system could detect 10 eggs of P. heterotremus per gram of cat feces. No fluorescence signal was observed when DNA purified from 16 other organisms or genomic DNA from cats and human beings were tested. Real-time FRET PCR yielded positive results for all fecal samples from 17 P. heterotremus\u2013infected cats and showed a negative relationship ( r = \u22120.852, P &lt; 0.001) between the number of parasite eggs in feces and the number of PCR cycles. The assay could detect genomic DNA from P. heterotremus, P. westermani, P. macrorchis, P. siamensis, P. harinasutai, and P. bangkokensis and can differentiate P. heterotremus from the other 5 species. The 6 Paragonimus species examined were divided into 4 groups by melting peak analysis. This assay can be useful for the detection of, and epidemiological studies on, P. heterotremus infection in endemic areas.\n                  <\/jats:p>","DOI":"10.1177\/1040638713497944","type":"journal-article","created":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T21:06:45Z","timestamp":1375132005000},"page":"620-626","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Application of a real-time fluorescence resonance energy transfer polymerase chain reaction assay with melting curve analysis for the detection of\n                    <i>Paragonimus heterotremus<\/i>\n                    eggs in the feces of experimentally infected cats"],"prefix":"10.1177","volume":"25","author":[{"given":"Chairat","family":"Tantrawatpan","sequence":"first","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penchom","family":"Janwan","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viraphong","family":"Lulitanond","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Witthaya","family":"Anamnart","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Research and Diagnostic Center for Emerging Infectious Diseases (Tantrawatpan, Intapan, Thanchomnang, Sanpool, Janwan, Lulitanond, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Departments of Parasitology (Intapan, Sanpool, Janwan, Maleewong), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Microbiology (Lulitanond), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"},{"name":"Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, Thailand (Tantrawatpan)"},{"name":"Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand (Thanchomnang)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,7,29]]},"reference":[{"key":"bibr1-1040638713497944","unstructured":"Beaver PC, Jung RC, Cupp EW: 1984, Examinations of specimens for parasites.\n                      In\n                      : Clinical parasitology, 9th ed., pp. 733\u2013758. Lea and Febiger, Philadelphia, PA."},{"key":"bibr2-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-71358-8_3"},{"key":"bibr3-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60149-9"},{"key":"bibr4-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2012.01.011"},{"key":"bibr5-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.05.023"},{"key":"bibr6-1040638713497944","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.12-0235"},{"key":"bibr7-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3357RM"},{"key":"bibr8-1040638713497944","unstructured":"Kawashima K, Sugiyama H, Ketudat P: 1989,\n                      Paragonimus\n                      infection in crabs in Thailand.\n                      In: Paragonimus\n                      in Asia: biology, genetic variation and speciation (\n                      Paragonimus\n                      Research Report, no. 2), pp. 75\u201379. Kyushu University, School of Health Sciences, Fukuoka, Japan."},{"key":"bibr9-1040638713497944","doi-asserted-by":"publisher","DOI":"10.3201\/eid1110.050614"},{"key":"bibr10-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1201\/9780203912065.ch37"},{"key":"bibr11-1040638713497944","doi-asserted-by":"publisher","DOI":"10.2307\/3284365"},{"key":"bibr12-1040638713497944","unstructured":"Miyazaki I: 1991, Paragonimiasis.\n                      In\n                      : An illustrated book of helminthic zoonoses, pp. 76\u2013146. International Medical Foundation of Japan, Tokyo."},{"key":"bibr13-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1968.11686533"},{"key":"bibr14-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2007.05.015"},{"key":"bibr15-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"bibr16-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-011-2804-7"},{"key":"bibr17-1040638713497944","first-page":"48","volume":"37","author":"Sugiyama H","year":"2006","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"bibr18-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762011000700008"},{"key":"bibr19-1040638713497944","unstructured":"Vajrasthira S: 1986, Paragonimiasis.\n                      In\n                      : Paragonimiasis, ed. Sucharit S, Areekul S, Sucharit P, pp. 98\u2013104. Krung Siam Press, Bangkok, Thailand."},{"key":"bibr20-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1054.1"},{"key":"bibr21-1040638713497944","unstructured":"World Health Organization (WHO): 1995, Control of foodborne trematode infections: report of a WHO study group (technical report series; 849). WHO, Geneva, Switzerland."},{"key":"bibr22-1040638713497944","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60364-4"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638713497944","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638713497944","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638713497944","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:17:47Z","timestamp":1777681067000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638713497944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,29]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1177\/1040638713497944"],"URL":"https:\/\/doi.org\/10.1177\/1040638713497944","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,29]]}}}