{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:16:18Z","timestamp":1790403378812,"version":"4.1.0"},"reference-count":15,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>Many commercial antibody detection enzyme-linked immunosorbent assay (ELISA) kits for Q fever utilize the Nine Mile (Montana tick) strain of Coxiella burnetii as antigen. An ELISA kit manufactured in France employs ovine placenta-sourced antigen and has been used in Europe. Sera from goats experiencing a Q fever abortion storm in the United States were used to compare the sensitivity and specificity of these 2 ELISA formats and the Q fever complement fixation test (CFT). Latent class estimates of sensitivity ranged from 97% to 100% with a specificity of 95\u2013100% for the 2 ELISA kits. Estimates for sensitivity and specificity of the CFT were 89% and 82%, respectively. There was not a significant increase in ELISA sensitivity observed with the ovine-sourced antigen kit in this study. Real-time polymerase chain reactions performed on a portion of the sera found that 15 out of 20 sera were congruent across 4 tests for positive and negative sera.<\/jats:p>","DOI":"10.1177\/1040638713517233","type":"journal-article","created":{"date-parts":[[2014,2,14]],"date-time":"2014-02-14T14:02:14Z","timestamp":1392386534000},"page":"141-145","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["<i>Coxiella burnetii<\/i>\n                    serology assays in goat abortion storm"],"prefix":"10.1177","volume":"26","author":[{"given":"Michelle P.","family":"Emery","sequence":"first","affiliation":[{"name":"U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA (Emery, Ostlund, McFarling, McGonigle)"},{"name":"Excet Inc., Springfield, VA (Ait Ichou, Ballin)"},{"name":"U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (Ait Ichou, Ballin)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eileen N.","family":"Ostlund","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA (Emery, Ostlund, McFarling, McGonigle)"},{"name":"Excet Inc., Springfield, VA (Ait Ichou, Ballin)"},{"name":"U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (Ait Ichou, Ballin)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Ait Ichou","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA (Emery, Ostlund, McFarling, McGonigle)"},{"name":"Excet Inc., Springfield, VA (Ait Ichou, Ballin)"},{"name":"U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (Ait Ichou, Ballin)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff D.","family":"Ballin","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA (Emery, Ostlund, McFarling, McGonigle)"},{"name":"Excet Inc., Springfield, VA (Ait Ichou, Ballin)"},{"name":"U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (Ait Ichou, Ballin)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"McFarling","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, Ames, IA (Emery, Ostlund, McFarling, McGonigle)"},{"name":"Excet Inc., Springfield, VA (Ait Ichou, Ballin)"},{"name":"U.S. Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD (Ait Ichou, 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