{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:36:26Z","timestamp":1789468586669,"version":"build-2803163510"},"reference-count":25,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T00:00:00Z","timestamp":1433980800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>To investigate the agreement between available serologic tests for the detection of antibodies against Anaplasma phagocytophilum and Borrelia burgdorferi, 50 serum samples from horses of unknown clinical status and at low risk for infection were tested. In addition to a point-of-care enzyme-linked immunosorbent assay (pocELISA), the evaluated tests included 2 indirect fluorescent antibody tests (IFATs) for antibodies against A. phagocytophilum and an IFAT, an ELISA confirmed with Western blot, and the Lyme multiplex assay for antibodies against B. burgdorferi. For each pair-wise comparison between serologic tests, the difference in the proportion of seropositive results as well as kappa and the prevalence-adjusted, bias-adjusted kappa were calculated. The proportion of seropositive results differed significantly in each pairwise comparison of tests for detection of antibodies against A. phagocytophilum, and between the pocELISA and IFAT as well as between the pocELISA and Lyme multiplex assay for detection of antibodies against B. burgdorferi. Agreement based on kappa varied from poor to fair while agreement was improved when evaluating prevalence-adjusted, bias-adjusted kappa. Lack of agreement may be explained by differences in methodology between the evaluated tests, cross-reactivity or false-positive and false-negative tests. In addition to the limitations of serologic test interpretation in the absence of clinical disease, this data suggest that screening of horses for exposure to tick-borne diseases in nonendemic areas may not be warranted.<\/jats:p>","DOI":"10.1177\/1040638715587548","type":"journal-article","created":{"date-parts":[[2015,6,11]],"date-time":"2015-06-11T23:06:51Z","timestamp":1434064011000},"page":"540-546","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison between available serologic tests for detecting antibodies against\n                    <i>Anaplasma phagocytophilum<\/i>\n                    and\n                    <i>Borrelia burgdorferi<\/i>\n                    in horses in Canada"],"prefix":"10.1177","volume":"27","author":[{"given":"Gili","family":"Schvartz","sequence":"first","affiliation":[{"name":"Departments of Large Animal Clinical Sciences (Schvartz, Epp, Lohmann), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Veterinary Pathology (Burgess), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Western College of Veterinary Medicine, and the Department of Biology (Chilton), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tasha","family":"Epp","sequence":"additional","affiliation":[{"name":"Departments of Large Animal Clinical Sciences (Schvartz, Epp, Lohmann), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Veterinary Pathology (Burgess), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Western College of Veterinary Medicine, and the Department of Biology (Chilton), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary J.","family":"Burgess","sequence":"additional","affiliation":[{"name":"Departments of Large Animal Clinical Sciences (Schvartz, Epp, Lohmann), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Veterinary Pathology (Burgess), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Western College of Veterinary Medicine, and the Department of Biology (Chilton), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil B.","family":"Chilton","sequence":"additional","affiliation":[{"name":"Departments of Large Animal Clinical Sciences (Schvartz, Epp, Lohmann), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Veterinary Pathology (Burgess), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Western College of Veterinary Medicine, and the Department of Biology (Chilton), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina L.","family":"Lohmann","sequence":"additional","affiliation":[{"name":"Departments of Large Animal Clinical Sciences (Schvartz, Epp, Lohmann), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Veterinary Pathology (Burgess), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Western College of Veterinary Medicine, and the Department of Biology (Chilton), University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,6,11]]},"reference":[{"key":"bibr1-1040638715587548","first-page":"174","volume":"37","author":"Berrington A","year":"1996","journal-title":"Can Vet J"},{"key":"bibr2-1040638715587548","first-page":"393","volume":"29","author":"Burgess B.","year":"1988","journal-title":"Can Vet J"},{"key":"bibr3-1040638715587548","first-page":"886","volume":"53","author":"Burgess H","year":"2012","journal-title":"Can Vet J"},{"key":"bibr4-1040638715587548","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2002.9695196"},{"key":"bibr5-1040638715587548","doi-asserted-by":"publisher","DOI":"10.1016\/0895-4356(93)90018-V"},{"key":"bibr6-1040638715587548","first-page":"590","volume-title":"MMWR Morb Mortal Wkly Rep","volume":"44","author":"Centers for Diseases Control and Prevention","year":"1995"},{"key":"bibr7-1040638715587548","first-page":"145","volume":"6","author":"Chandrashekar R","year":"2008","journal-title":"Int J Appl Res Vet Med"},{"key":"bibr8-1040638715587548","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.02.006"},{"key":"bibr9-1040638715587548","first-page":"97","volume-title":"Veterinary Epidemiologic Research","volume":"2009","author":"Dohoo I","edition":"2"},{"key":"bibr10-1040638715587548","unstructured":"Foley JE, et al. 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