{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:37:03Z","timestamp":1777930623701,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>Cats are infected by Anaplasma phagocytophilum and Borrelia burgdorferi when exposed to infected Ixodes scapularis (black-legged ticks). The purpose of our study was to allow wild-caught I. scapularis to feed on healthy research cats ( n = 4) and temporally evaluate for A. phagocytophilum DNA in blood by a polymerase chain reaction (PCR) assay as well as for antibody responses to the B. burgdorferi C6 peptide, to the A. phagocytophilum P44 peptide, and to a novel A. phagocytophilum peptide (P44-4). Prior to I. scapularis infestation, all cats were negative for antibodies against both organisms based on a kit optimized for dog serum, and negative for A. phagocytophilum DNA in blood using a conventional PCR assay. Using the pre-infestation samples, an enzyme-linked immunosorbent assay for detecting antibodies against the P44-4 peptide was optimized. Cats were infested with wild-caught I. scapularis for 7 days. Genomic DNA of A. phagocytophilum was amplified from the blood before antibodies were detected in all 4 cats. Antibodies against the C6 peptide, P44 peptide, and P44-4 peptide were detected in the sera of all 4 cats. Antibodies against P44-4 were detected prior to those against P44 in 3 out of 4 cats. The results suggest that a PCR assay should be considered in acutely ill cats with suspected anaplasmosis that are seronegative.<\/jats:p>","DOI":"10.1177\/1040638715593598","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T22:32:24Z","timestamp":1436999544000},"page":"522-525","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":42,"title":["Evidence of\n                    <i>Anaplasma phagocytophilum<\/i>\n                    and\n                    <i>Borrelia burgdorferi<\/i>\n                    infection in cats after exposure to wild-caught adult\n                    <i>Ixodes scapularis<\/i>"],"prefix":"10.1177","volume":"27","author":[{"given":"Michael R.","family":"Lappin","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin)"},{"name":"IDEXX Laboratories Inc., Portland, ME (Chandrashekar, Stillman, Liu)"},{"name":"Center for Vector-Borne Disease, University of Rhode Island, Kingston, RI (Mather)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramaswamy","family":"Chandrashekar","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin)"},{"name":"IDEXX Laboratories Inc., Portland, ME (Chandrashekar, Stillman, Liu)"},{"name":"Center for Vector-Borne Disease, University of Rhode Island, Kingston, RI (Mather)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brett","family":"Stillman","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin)"},{"name":"IDEXX Laboratories Inc., Portland, ME (Chandrashekar, Stillman, Liu)"},{"name":"Center for Vector-Borne Disease, University of Rhode Island, Kingston, RI (Mather)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayou","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin)"},{"name":"IDEXX Laboratories Inc., Portland, ME (Chandrashekar, Stillman, Liu)"},{"name":"Center for Vector-Borne Disease, University of Rhode Island, Kingston, RI (Mather)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas N.","family":"Mather","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Colorado State University, Fort Collins, CO (Lappin)"},{"name":"IDEXX Laboratories Inc., Portland, ME (Chandrashekar, Stillman, Liu)"},{"name":"Center for Vector-Borne Disease, University of Rhode Island, Kingston, RI 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