{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:37:05Z","timestamp":1777930625976,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T00:00:00Z","timestamp":1437436800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>Toxoplasma gondii can infect almost all warm-blooded animals, and many serological methods have been developed to detect T. gondii infection in a variety of animal species. In the present study, the seroprevalence of T. gondii infection in farmed mink in northeast China was determined using the modified agglutination test (MAT), a Western blot (WB), and 3 enzyme-linked immunosorbent assays (ELISAs) with protein A\/G conjugate, using either of 2 recombinant dense granule antigens, GRA1 and GRA7, or Toxoplasma soluble antigens (TSA). There was no significant difference between the detection results of the GRA1-, GRA7-, and TSA-ELISAs and WB (McNemar chi-square, P &gt; 0.05), but a significant difference was observed between MAT and WB ( P &lt; 0.05). A near perfect agreement (97.0%) was found between the GRA7-ELISA and WB (\u03ba = 0.83), and a substantial agreement (92.4\u201393.1%) was observed in the TSA- and GRA1-ELISAs (\u03ba = 0.68\u20130.73). The GRA7-ELISA showed the highest sensitivity and specificity, and the lowest false-positive and negative rates, while the MAT gave both a low sensitivity and frequent false positives in comparison to the WB. Receiver operating characteristic analysis revealed the largest area under curve of 0.85 (95% confidence interval: 0.74\u20130.96), and the highest relative sensitivity (72.7%) and specificity (99.0%) for a cutoff value of 0.19 in the GRA7-ELISA. These results indicate that the GRA7-ELISA is suitable for detection of T. gondii infection in mink and that MAT should be used with caution.<\/jats:p>","DOI":"10.1177\/1040638715596033","type":"journal-article","created":{"date-parts":[[2015,7,21]],"date-time":"2015-07-21T22:40:18Z","timestamp":1437518418000},"page":"616-620","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["A comparative study of\n                    <i>Toxoplasma gondii<\/i>\n                    seroprevalence in mink using a modified agglutination test, a Western blot, and enzyme-linked immunosorbent assays"],"prefix":"10.1177","volume":"27","author":[{"given":"Yi","family":"Gu","sequence":"first","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, Wei)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zedong","family":"Wang","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, Wei)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufeng","family":"Cai","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, Wei)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxing","family":"Li","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, Wei)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Wei","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, 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(Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, Wei)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Liu","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin Province, People\u2019s Republic of China (Gu, Wang, Shang, Li, Liu)"},{"name":"College of Life Science, Jilin Agricultural University, Changchun, Jilin Province, People\u2019s Republic of China (Cai, Li, 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