{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:37:23Z","timestamp":1777930643625,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,5,6]],"date-time":"2016-05-06T00:00:00Z","timestamp":1462492800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p>\n                    Rabies has been an enigmatic disease because microscopic findings in central nervous system tissues do not always correlate well with the severity of the clinical illness. Immunohistochemical staining of the calcium-binding protein calbindin (specifically CbD28k) seems to be the technique most used to identify Purkinje neurons under normal and pathological conditions. In the present work, we evaluated CbD28k immunoreactivity in the cerebellar cortex of normal and natural Rabies virus (RABV)-infected cattle. We examined brains from 3 normal cows and from 6 crossbreed cattle with a histologic diagnosis of rabies. Samples were taken from the cerebral cortex, cerebellum, hippocampus, and brainstem. Immunohistochemistry was carried out using the following primary antibodies: anti-RABV, anti-GFAP, and anti-CbD28k. In the cerebellar cortex, RABV infection caused the loss of CbD28k immunostaining in Purkinje cells; some large interneurons in the granular layer maintained their positive CbD28k immunoreaction. The identification of this loss of CbD28k reactivity in cerebellar Purkinje cells of RABV-infected cattle presents a potentially valuable tool to explore the impairment of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    homeostasis. In addition, this may become a useful method to identify specific molecular alterations associated with the higher prevalence of Negri bodies in Purkinje cells of cattle. Furthermore, we detected the presence of rabies viral antigens in different regions of the central nervous system, accompanied by microglial proliferation and mild reactive astrogliosis.\n                  <\/jats:p>","DOI":"10.1177\/1040638716644485","type":"journal-article","created":{"date-parts":[[2016,5,7]],"date-time":"2016-05-07T03:04:39Z","timestamp":1462590279000},"page":"361-368","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Calbindin D28k distribution in neurons and reactive gliosis in cerebellar cortex of natural\n                    <i>Rabies virus<\/i>\n                    \u2013infected cattle"],"prefix":"10.1177","volume":"28","author":[{"given":"Jos\u00e9 Manuel","family":"Verdes","sequence":"first","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiano Jos\u00e9 Ferreira","family":"de Sant\u2019Ana","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Sabalsagaray","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kosuke","family":"Okada","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldo","family":"Calliari","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Antonio","family":"Mora\u00f1a","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio Severo Lombardo","family":"de Barros","sequence":"additional","affiliation":[{"name":"Departments of Pathology (Verdes, Sabalsagaray, Okada, Mora\u00f1a), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Molecular and Cellular Biology (Biophysics) (Verdes, Calliari), Faculty of Veterinary, University of the Republic, Montevideo, Uruguay"},{"name":"Laboratory of Veterinary Pathology Diagnosis, University of Brasilia, Brasilia, DF, Brazil (Sant\u2019Ana)"},{"name":"Laboratory of Veterinary Pathology, Federal University of Santa Mar\u00eda, Santa Maria, Rio Grande do Sul, Brazil (de Barros)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,5,6]]},"reference":[{"key":"bibr1-1040638716644485","first-page":"215","volume-title":"Rabies: Scientific Basis of the Disease and its Management","volume":"2013","author":"Banyard AC","edition":"3"},{"key":"bibr2-1040638716644485","unstructured":"Barros CSL, In: Barros CSL, ed. Doen\u00e7as do sistema nervoso de bovinos no Brasil [Nervous system diseases of cattle in Brazil]. S\u00e3o Paulo, Brazil: Vall\u00e9e, 2006:21\u201328. Portuguese."},{"key":"bibr3-1040638716644485","doi-asserted-by":"publisher","DOI":"10.1080\/14734220310022289"},{"key":"bibr4-1040638716644485","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00423-2"},{"key":"bibr5-1040638716644485","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.07.005"},{"key":"bibr6-1040638716644485","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90091-H"},{"key":"bibr7-1040638716644485","unstructured":"Easton C, ed. Identificaci\u00f3n de agentes infecciosos en encefalitis de bovinos de Uruguay [Identification of infectious agents in encephalitides of cattle in Uruguay]. Montevideo, Uruguay: Promotion Fund for Agricultural Technology (FPTA)-National Agricultural Research Institute (INIA), 2012:9\u201328. FPTA No. 40. 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