{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T01:02:05Z","timestamp":1788742925740,"version":"build-2803163510"},"reference-count":63,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T00:00:00Z","timestamp":1487203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Encephalitozoon cuniculi is an important microsporidian pathogen that is considered an emergent, zoonotic, and opportunistic. It infects both domestic and laboratory rabbits, generating severe chronic interstitial and granulomatous nephritis with fibrosis and granulomatous encephalitis. Encephalitozoonosis is diagnosed in paraffin-embedded sections by examining the spores in the host tissues. The spores are difficult to observe when the samples are stained with hematoxylin and eosin (H&amp;E), particularly when there is an inflammatory reaction and tissue damage. The spores are easily mistaken for other microorganisms, such as fungi (yeasts), protozoa, and bacteria. In our study, we used kidney samples from E. cuniculi\u2013positive rabbits and employed 14 recommended histologic stains for detecting microsporidia spores: alcian blue, calcofluor white, Giemsa, Gram, Grocott, H&amp;E, Luna, Luxol fast blue, Masson trichrome, modified trichrome stain (MTS), periodic acid\u2013Schiff reaction (PAS), Van Gieson, Warthin\u2013Starry (WS), and Ziehl\u2013Neelsen (ZN).We concluded that MTS and Gram stain, detected by light microscopy, and calcofluor white stain, detected by ultraviolet light microscopy, are the best stains for detecting spores of E. cuniculi in paraffin-embedded tissues from infected rabbits. These stains were superior to WS, ZN, Giemsa, and PAS for identifying spores without background \u201cnoise\u201d or monochromatic interference. Also, they allow individual spores to be discerned in paraffin-embedded tissues. MTS allows observation of the polar tube, polaroplast, and posterior vacuole, the most distinctive parts of the spore.<\/jats:p>","DOI":"10.1177\/1040638716668559","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T07:26:07Z","timestamp":1487229967000},"page":"269-277","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Histochemical study of\n                    <i>Encephalitozoon cuniculi<\/i>\n                    spores in the kidneys of naturally infected New Zealand rabbits"],"prefix":"10.1177","volume":"29","author":[{"given":"Luis E.","family":"Rodr\u00edguez-Tovar","sequence":"first","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Zoonosis y Enfermedades Emergentes (Rodr\u00edguez, Villarreal, Nev\u00e1rez, Castillo, Rodr\u00edguez, Navarro, Trejo), Department of Immunology, FMVZ-UANL, Campus de Ciencias Agropecuarias, General Escobedo, Nuevo Le\u00f3n, Mexico"},{"name":"Cuerpo Acad\u00e9mico de Epidemiolog\u00eda Veterinaria (Zarate), Department of Immunology, FMVZ-UANL, Campus de Ciencias Agropecuarias, General Escobedo, Nuevo Le\u00f3n, Mexico"},{"name":"Cuerpo Acad\u00e9mico de Patobiolog\u00eda (Hern\u00e1ndez), Department of Immunology, FMVZ-UANL, Campus de Ciencias Agropecuarias, General Escobedo, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Villarreal-Marroqu\u00edn","sequence":"additional","affiliation":[{"name":"Cuerpo Acad\u00e9mico de Zoonosis y Enfermedades Emergentes (Rodr\u00edguez, Villarreal, Nev\u00e1rez, Castillo, Rodr\u00edguez, Navarro, Trejo), Department of Immunology, FMVZ-UANL, Campus de Ciencias Agropecuarias, General Escobedo, Nuevo Le\u00f3n, Mexico"},{"name":"Cuerpo Acad\u00e9mico de Epidemiolog\u00eda Veterinaria (Zarate), Department of Immunology, FMVZ-UANL, Campus de Ciencias Agropecuarias, General Escobedo, Nuevo Le\u00f3n, 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