{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T17:12:04Z","timestamp":1784308324843,"version":"3.55.0"},"reference-count":26,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T00:00:00Z","timestamp":1492560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>We report the development and field validation of 2 ELISAs for the detection of Ancylostoma caninum or Toxocara canis coproantigens in the feces of dogs with experimental and natural infections, and evidence of cross-reactivity with respective feline counterparts. A. caninum\u2013specific coproantigens were detected in feces of experimentally infected dogs starting at 9 d post-infection (dpi), whereas eggs were not seen until 23 dpi. T. canis\u2013specific coproantigens were detected in 3 of 5 experimentally infected dogs by 31 dpi, and 4 of the 5 animals by 38 dpi. T. canis eggs were seen in feces of 4 of the 5 animals by 38 dpi. One dog had delayed coproantigen detection and low egg output. Additionally, 817 canine and 183 feline fecal samples from naturally infected animals tested by flotation were subjected to coproantigen ELISA testing. Of these 1,000 canine and feline samples, 13 and 23 samples, respectively, were positive for \u201chookworm\u201d or \u201croundworm\u201d eggs; 19 and 26 samples were ELISA positive, respectively. The T. canis ELISA detected T. cati coproantigen in cat fecal samples. Discrepant ELISA and flotation results were obtained for 16 hookworm- and 13 roundworm-positive samples. Re-examination of the egg-positive, ELISA-negative samples indicated several instances of possible misidentification or coprophagy, whereas detection of antigen in samples without egg observations is likely a reflection of true infection status with egg shedding below detection levels. There is good indication, based on accumulated field data, that these antigen tests also detect other hookworm and ascarid species.<\/jats:p>","DOI":"10.1177\/1040638717706098","type":"journal-article","created":{"date-parts":[[2017,4,20]],"date-time":"2017-04-20T01:49:12Z","timestamp":1492652952000},"page":"645-653","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":57,"title":["Enzyme-linked immunosorbent assays for coproantigen detection of\n                    <i>Ancylostoma caninum<\/i>\n                    and\n                    <i>Toxocara canis<\/i>\n                    in dogs and\n                    <i>Toxocara cati<\/i>\n                    in cats"],"prefix":"10.1177","volume":"29","author":[{"given":"David A.","family":"Elsemore","sequence":"first","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinming","family":"Geng","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Cote","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Hanna","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Araceli","family":"Lucio-Forster","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwight D.","family":"Bowman","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Westbrook, ME (Elsemore, Geng, Cote, Hanna)"},{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY (Bowman, Lucio-Forster)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,4,19]]},"reference":[{"key":"bibr1-1040638717706098","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90530-7"},{"key":"bibr2-1040638717706098","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.02.016"},{"key":"bibr3-1040638717706098","volume":"52","author":"Bowman DD","year":"1991","journal-title":"Am J Vet Res"},{"key":"bibr4-1040638717706098","unstructured":"Bowman DD, et al. Toxocara cati (Schrank, 1788). In: The Nematodes. Feline Clinical Parasitology. 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