{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T15:01:58Z","timestamp":1784214118049,"version":"3.55.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T00:00:00Z","timestamp":1496620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:p>We developed a real-time (rt)PCR assay based on TaqMan probe technology for the specific detection of canine adenovirus 1 (CAdV-1). The assay is able to detect three 50% tissue culture infectious dose\/mL in CAdV-1\u2013containing cell culture supernatant. Viral genomes were not amplified of canine adenovirus 2 or of several bovine, porcine, and avian adenoviruses. In silico analysis provided no indication of amplification of other heterologous genomes. The sensitivity of the real-time assay exceeded that of a conventional gel-based CAdV-1 PCR by a factor of 100. Following the integration of the novel PCR into the Hessian wildlife-monitoring program, CAdV-1 DNA was detected in none of the tested raccoons ( n = 48) but in 11 of 97 foxes.<\/jats:p>","DOI":"10.1177\/1040638717712331","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T08:33:46Z","timestamp":1496651626000},"page":"741-746","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Detection of canine adenovirus 1 in red foxes (\n                    <i>Vulpes vulpes<\/i>\n                    ) and raccoons (\n                    <i>Procyon lotor<\/i>\n                    ) in Germany with a TaqMan real-time PCR assay"],"prefix":"10.1177","volume":"29","author":[{"given":"Silke","family":"Hechinger","sequence":"first","affiliation":[{"name":"Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svenja","family":"Scheffold","sequence":"additional","affiliation":[{"name":"Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Hamann","sequence":"additional","affiliation":[{"name":"Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Zsch\u00f6ck","sequence":"additional","affiliation":[{"name":"Hessian State Laboratory, Department of Veterinary Medicine, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,6,5]]},"reference":[{"key":"bibr1-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"bibr2-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.11.015"},{"key":"bibr3-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2014.10.010"},{"key":"bibr4-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2015.05.009"},{"key":"bibr5-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006058"},{"key":"bibr6-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1638\/2013-0286.1"},{"key":"bibr7-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1177\/104063879801000402"},{"key":"bibr8-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2006.11.009"},{"key":"bibr9-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2008.02.006"},{"key":"bibr10-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2016.03.011"},{"key":"bibr11-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.05.004"},{"key":"bibr12-1040638717712331","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-43.4.734"},{"key":"bibr13-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006417203856"},{"key":"bibr14-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1638\/05-123.1"},{"key":"bibr15-1040638717712331","first-page":"253","volume":"44","author":"Kiss I","year":"1996","journal-title":"Acta Vet Hung"},{"key":"bibr16-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-015-1074-8"},{"key":"bibr17-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1136\/vr.b4763"},{"key":"bibr18-1040638717712331","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.03.028"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638717712331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638717712331","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638717712331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:20:49Z","timestamp":1777681249000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638717712331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,5]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1177\/1040638717712331"],"URL":"https:\/\/doi.org\/10.1177\/1040638717712331","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,5]]}}}