{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:38:18Z","timestamp":1777930698610,"version":"3.51.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>We compared a qualitative in-clinic (IC)-PCR for the detection of Mycoplasma haemofelis DNA with the results of a commercial qualitative laboratory-based, conventional (c)PCR. In order to determine the specificity of both tests, Bartonella spp. samples were included. Forty-three previously tested blood samples with known PCR results for hemoplasmas and Bartonella spp. were selected. The samples were split between 2 laboratories. At the first laboratory, DNA was purified and run on 2 cPCR assays for the detection of hemoplasmas and Bartonella spp. At the second laboratory, DNA was purified using 2 purification protocols and both run in the IC-PCR assay. The cPCR results confirmed that 18 samples were positive for M. haemofelis, 5 for \u2018 Candidatus M. haemominutum\u2019, 8 for Bartonella henselae, 2 for Bartonella clarridgeiae, and 10 were negative for both genera. No mixed infections were observed. The IC-PCR assay for the detection of M. haemofelis had a sensitivity of 94.4% and specificity of 96%, when using the same DNA purification method as the first laboratory. Using the second purification method, the sensitivity of the IC-PCR assay was 77.8% and specificity was 96%. Bartonella species were not detected by the IC-PCR M. haemofelis assay. The IC-PCR assay decreased the amount of time to final result compared to a cPCR assay.<\/jats:p>","DOI":"10.1177\/1040638717729187","type":"journal-article","created":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T09:13:24Z","timestamp":1505380404000},"page":"140-143","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Amplification of\n                    <i>Mycoplasma haemofelis<\/i>\n                    DNA by a PCR for point-of-care use"],"prefix":"10.1177","volume":"30","author":[{"given":"Jennifer","family":"Hawley","sequence":"first","affiliation":[{"name":"Biogal, Galed Labs. Acs Ltd., Kibbutz Galed, Israel (Maurice, Yaaran)"},{"name":"Center for Companion Animal Studies, Colorado State University, Fort Collins, CO (Hawley, Lappin)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tal","family":"Yaaran","sequence":"additional","affiliation":[{"name":"Biogal, Galed Labs. Acs Ltd., Kibbutz Galed, Israel (Maurice, Yaaran)"},{"name":"Center for Companion Animal Studies, Colorado State University, Fort Collins, CO (Hawley, Lappin)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Maurice","sequence":"additional","affiliation":[{"name":"Biogal, Galed Labs. Acs Ltd., Kibbutz Galed, Israel (Maurice, Yaaran)"},{"name":"Center for Companion Animal Studies, Colorado State University, Fort Collins, CO (Hawley, Lappin)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Lappin","sequence":"additional","affiliation":[{"name":"Biogal, Galed Labs. Acs Ltd., Kibbutz Galed, Israel (Maurice, Yaaran)"},{"name":"Center for Companion Animal Studies, Colorado State University, Fort Collins, CO (Hawley, Lappin)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,9,14]]},"reference":[{"key":"bibr1-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.2010.00569.x"},{"key":"bibr2-1040638717729187","doi-asserted-by":"publisher","DOI":"10.2460\/javma.236.8.869"},{"key":"bibr3-1040638717729187","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050931"},{"key":"bibr4-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2006.05.003"},{"key":"bibr5-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1717-1722.2000"},{"key":"bibr6-1040638717729187","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.604"},{"key":"bibr7-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2010.03.011"},{"key":"bibr8-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1053\/jfms.2001.0155"},{"key":"bibr9-1040638717729187","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2581-2585.2005"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638717729187","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638717729187","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638717729187","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:21:05Z","timestamp":1777681265000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638717729187"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,14]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1177\/1040638717729187"],"URL":"https:\/\/doi.org\/10.1177\/1040638717729187","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,14]]}}}