{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:38:48Z","timestamp":1777930728138,"version":"3.51.4"},"reference-count":37,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2019,9,28]],"date-time":"2019-09-28T00:00:00Z","timestamp":1569628800000},"content-version":"vor","delay-in-days":365,"URL":"http:\/\/www.sagepub.com\/licence-information-for-chorus"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2013-67015-21314"],"award-info":[{"award-number":["2013-67015-21314"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["NC-1192 Multi-State Research Project"],"award-info":[{"award-number":["NC-1192 Multi-State Research Project"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>Histophilus somni is an opportunistic pathogen responsible for respiratory and systemic diseases of cattle and sheep. Rapid and accurate detection of H. somni is essential to distinguish H. somni from other potential pathogens for proper control and treatment of infections. Nanomaterial optical fiber biosensors (NOFS) recognize analyte interactions, such as DNA hybridization, with high specificity and sensitivity, and were applied to detect H. somni DNA in culture and clinical samples. An ionic self-assembled multilayer (ISAM) film was fabricated on a long-period grating optical fiber, and a biotinylated, nucleotide probe complementary to the H. somni 16S rDNA gene was coupled to the ISAM film. Exposure of the ISAM::probe to \u2a7e100 killed cells of H. somni strain 2336 without DNA amplification resulted in attenuation of light transmission of \u2a7e9.4%. Exposure of the complexed fiber to Escherichia coli or non\u2013 H. somni species of Pasteurellaceae reduced light transmission by \u2a7d3.4%. Exposure of the ISAM::probe to blood, bronchoalveolar fluid, or spleen from mice or calves infected with H. somni resulted in \u2a7e24.3% transmission attenuation. The assay correctly detected all 6 strains of H. somni tested from culture, or tissues from 3 separate mice and calves tested in duplicate. Six heterologous strains (representing 6 genera) reacted at below the cutoff value of 4.87% attenuation of light transmission. NOFS detected at least 100 H. somni cells without DNA amplification within 45\u2004min with high specificity. Although different fibers could vary in signal sensitivity, this did not affect the sensitivity or specificity of the assay.<\/jats:p>","DOI":"10.1177\/1040638718803665","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T07:57:42Z","timestamp":1538121462000},"page":"821-829","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of\n                    <i>Histophilus somni<\/i>\n                    by a nanomaterial optical fiber biosensor assay"],"prefix":"10.1177","volume":"30","author":[{"given":"Aloka B.","family":"Bandara","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine (Bandara, Inzana)"},{"name":"Department of Physics, College of Science (Zuo, McCutcheon, Heflin)"},{"name":"Virginia Tech, Blacksburg, VA; Department of Electrical and Computer Engineering, Boston University, Boston, MA (Ramachandran)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziwei","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine (Bandara, Inzana)"},{"name":"Department of Physics, College of Science (Zuo, McCutcheon, Heflin)"},{"name":"Virginia Tech, Blacksburg, VA; Department of Electrical and Computer Engineering, Boston University, Boston, MA (Ramachandran)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly","family":"McCutcheon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine (Bandara, Inzana)"},{"name":"Department of Physics, College of Science (Zuo, McCutcheon, Heflin)"},{"name":"Virginia Tech, Blacksburg, VA; Department of Electrical and Computer Engineering, Boston University, Boston, MA 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