{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:56:38Z","timestamp":1787316998398,"version":"build-2736575974"},"reference-count":33,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T00:00:00Z","timestamp":1544572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"ANSES, French Agency for Food, Environmental and Occupational Health and Safety"},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:p>\n                    Rabies is diagnosed postmortem in animals, based on tests prescribed by the World Organization for Animal Health (OIE), such as the fluorescent antibody test, the direct rapid immunohistochemistry test, or pan-lyssavirus PCR assays. Several reverse-transcription real-time PCR (RT-rtPCR) methods have been developed and validated for rapid and accurate detection of lyssaviruses. We evaluated the performance of 6 TaqMan RT-rtPCR kits using different commercial master mixes and 2 real-time thermocyclers. Changing the master mix overall did not influence the TaqMan RT-rtPCR performance, regardless of the thermocycler used. The limits of detection at the 95% confidence level were 18.1\u201325.8 copies\/\u00b5L for the Rotor-Gene Q MDx thermocycler and 16.7\u201321.5 for the Mx3005P thermocycler. Excellent repeatability was demonstrated for rabies virus (RABV) RNA samples of 100, 50, and 25 copies\/\u00b5L regardless of the thermocycler used. RABV field samples ( n = 35) isolated worldwide gave positive results using the most efficient of the 6 kits tested, with a copy number of 6.03 \u00d7 10\n                    <jats:sup>2<\/jats:sup>\n                    to 6.78 \u00d7 10\n                    <jats:sup>7<\/jats:sup>\n                    RNA copies per reaction. The TaqMan RT-rtPCR assay provides sensitive and rapid amplification of RABV RNA.\n                  <\/jats:p>","DOI":"10.1177\/1040638718818223","type":"journal-article","created":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T01:55:30Z","timestamp":1544666130000},"page":"47-57","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of six TaqMan RT-rtPCR kits on two thermocyclers for the reliable detection of rabies virus RNA"],"prefix":"10.1177","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9122-9027","authenticated-orcid":false,"given":"Evelyne","family":"Picard-Meyer","sequence":"first","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Peytavin de Garam","sequence":"additional","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Luc","family":"Schereffer","sequence":"additional","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Robardet","sequence":"additional","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Cliquet","sequence":"additional","affiliation":[{"name":"ANSES Nancy Laboratory for Rabies and Wildlife, Malz\u00e9ville, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,12,12]]},"reference":[{"key":"bibr1-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-328"},{"key":"bibr2-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.3.519-523.1989"},{"key":"bibr3-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2012.10.003"},{"key":"bibr4-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01256-10"},{"key":"bibr5-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004812"},{"key":"bibr6-1040638718818223","first-page":"88","volume-title":"Laboratory Techniques in Rabies","author":"Dean DJ","year":"1996","edition":"4"},{"key":"bibr7-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.1.165-256.2006"},{"key":"bibr8-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.03.014"},{"key":"bibr9-1040638718818223","first-page":"264","volume":"125","author":"Fischer M","year":"2012","journal-title":"Berl Munch Tierarztl Wochenschr"},{"key":"bibr10-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)62707-5"},{"key":"bibr11-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197074"},{"key":"bibr12-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.07.002"},{"key":"bibr13-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2762-2766.1997"},{"key":"bibr14-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00612-10"},{"key":"bibr15-1040638718818223","first-page":"360","volume":"83","author":"Knobel DL","year":"2005","journal-title":"Bull World Health Organ"},{"key":"bibr16-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-012-9445-z"},{"key":"bibr17-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-016-2797-3"},{"key":"bibr18-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2014.06.024"},{"key":"bibr19-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21547"},{"key":"bibr20-1040638718818223","first-page":"87","volume-title":"A-Z of Quantitative PCR","author":"Pfaffl M","year":"2004"},{"key":"bibr21-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/839518"},{"key":"bibr22-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0444-2015"},{"key":"bibr23-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005540"},{"key":"bibr24-1040638718818223","unstructured":"Siegel S, Castellan NJ. Non Parametric Statistics for the Behavioural Sciences. New York: MacGraw Hill, 1988:213\u2013214."},{"key":"bibr25-1040638718818223","first-page":"39","volume":"27","author":"Speers DJ.","year":"2006","journal-title":"Clin Biochem Rev"},{"key":"bibr26-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2010.08.004"},{"key":"bibr27-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1086\/314015"},{"key":"bibr28-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/256175"},{"key":"bibr29-1040638718818223","doi-asserted-by":"publisher","DOI":"10.4103\/2277-9175.127998"},{"key":"bibr30-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005258"},{"key":"bibr31-1040638718818223","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2786-2792.2005"},{"key":"bibr32-1040638718818223","unstructured":"World Organization for Animal Health (OIE). Chapter 3.6.3. Development and optimisation of nucleic acid detection assays. In: OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris, France: OIE, 2018:1\u201311."},{"key":"bibr33-1040638718818223","unstructured":"World Organization for Animal Health (OIE). Chapter 2.1.17. Rabies (infection with rabies and other lyssaviruses). In: OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris, France: OIE, 2018:1\u201335."}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638718818223","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638718818223","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638718818223","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:22:08Z","timestamp":1777681328000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638718818223"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,12]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1177\/1040638718818223"],"URL":"https:\/\/doi.org\/10.1177\/1040638718818223","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,12]]}}}