{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:03Z","timestamp":1777930743649,"version":"3.51.4"},"reference-count":38,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T00:00:00Z","timestamp":1554336000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>\n                    Malassezia pachydermatis is part of the normal microbiota of canine skin and external ear canal, and is also associated with otitis externa in dogs. Laboratory detection of Malassezia otitis relies on the presence of elevated numbers of the yeast on cytologic examination of otic exudate. Although cytology has high specificity, it has low sensitivity, resulting in false-negatives and posing a challenge for clinicians to accurately diagnose Malassezia otitis. We developed a quantitative PCR (qPCR) to detect and quantify M. pachydermatis yeasts and validate the method with swabs from external ear canals of dogs. Our qPCR uses the \u03b2-tubulin gene, a single-copy gene, as a target. The limit of quantification was established as 0.18\u2009ng\/reaction, equivalent to 2.0 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    genome equivalents (gEq). Swabs from healthy dogs yielded quantification values of \u22642.7 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    \u2009gEq in the qPCR, whereas swabs from dogs with otitis yielded quantification values of \u22652.5 \u00d7 10\n                    <jats:sup>5<\/jats:sup>\n                    \u2009gEq. Our qPCR assay provides accurate quantification of M. pachydermatis yeasts from swab samples from dogs, is more sensitive than cytology, and could be used to monitor response to treatment. Our assay could also be valuable in a research setting to better understand the pathogenesis of M. pachydermatis.\n                  <\/jats:p>","DOI":"10.1177\/1040638719840686","type":"journal-article","created":{"date-parts":[[2019,4,4]],"date-time":"2019-04-04T03:20:39Z","timestamp":1554348039000},"page":"440-447","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Quantification of\n                    <i>Malassezia pachydermatis<\/i>\n                    by real-time PCR in swabs from the external ear canal of dogs"],"prefix":"10.1177","volume":"31","author":[{"given":"Laura","family":"Puig","sequence":"first","affiliation":[{"name":"Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Aut\u00f2noma de Barcelona, Bellaterra, Catalonia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8404-0688","authenticated-orcid":false,"given":"Gemma","family":"Castell\u00e1","sequence":"additional","affiliation":[{"name":"Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Aut\u00f2noma de Barcelona, Bellaterra, Catalonia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Javier","family":"Caba\u00f1es","sequence":"additional","affiliation":[{"name":"Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Aut\u00f2noma de Barcelona, Bellaterra, Catalonia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,4,4]]},"reference":[{"key":"bibr1-1040638719840686","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2010.00913.x"},{"key":"bibr2-1040638719840686","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2003.10.005"},{"key":"bibr3-1040638719840686","first-page":"32","volume":"24","author":"Black J","year":"2013","journal-title":"J Biomol 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