{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:11Z","timestamp":1777930751457,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T00:00:00Z","timestamp":1556841600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>\n                    The European Community\u2019s leukosis key (EC key) is a well-known hematologic method for detecting bovine leukemia virus (BLV) infection in dairy cattle. The key identifies infected cattle with persistent lymphocytosis via a combination of lymphocyte count (LC) and age. Using the EC key to identify BLV-infected Japanese black (JB) cattle is problematic, however, given the inherently lower LCs of JB cattle compared to dairy cattle. We analyzed the LC in BLV-positive and -negative JB cattle and estimated LC cutoff values by age using receiver operating characteristic curve analysis. Among the 716 JB blood samples collected, 452 (63%) JB cattle were confirmed as BLV-positive by an antibody ELISA for \u22651-y-old cattle and by real-time PCR for &lt;1-y-old cattle. The cutoff values for the LC in each age group were calculated as 6.3 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for &lt;1\u2009y, 5.9 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for 1 to &lt;2\u2009y, 5.5 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for 2 to &lt;3 y, 4.5 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for 3 to &lt;6\u2009y, 4.3 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for 6 to \u226410\u2009y, and 3.7 \u00d7 10\n                    <jats:sup>9<\/jats:sup>\n                    \/L for &gt;10\u2009y. The sensitivity and specificity of the estimated cutoff values were 0.49 (95% confidence interval: 0.44\u20130.53) and 0.81 (0.75\u20130.85), whereas those of the EC key were 0.20 (0.16\u20130.24) and 0.99 (0.97\u20131.00). Our LC cutoff values for screening JB cattle for BLV infection appear to be preferable to those of the EC key.\n                  <\/jats:p>","DOI":"10.1177\/1040638719848449","type":"journal-article","created":{"date-parts":[[2019,5,3]],"date-time":"2019-05-03T06:13:15Z","timestamp":1556863995000},"page":"568-571","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["A hematologic key for bovine leukemia virus screening in Japanese black cattle"],"prefix":"10.1177","volume":"31","author":[{"given":"Masataka","family":"Akagami","sequence":"first","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Oya","sequence":"additional","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Kashima","sequence":"additional","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Seki","sequence":"additional","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinao","family":"Ouchi","sequence":"additional","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4814-0945","authenticated-orcid":false,"given":"Yoko","family":"Hayama","sequence":"additional","affiliation":[{"name":"Ibaraki Prefecture Kenpoku Livestock Hygiene Service Center, Ibaraki, Japan (Akagami, Oya, Kashima, Seki, Ouchi)"},{"name":"Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan (Hayama)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,5,3]]},"reference":[{"key":"bibr1-1040638719848449","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.74.5.744"},{"key":"bibr2-1040638719848449","first-page":"215","volume":"39","author":"Bendixen HJ.","year":"1973","journal-title":"Bibl Haematol"},{"key":"bibr3-1040638719848449","first-page":"49","volume":"29","author":"Flensburg JC","year":"1977","journal-title":"Nord 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Evaluation of diagnostic tests. In: Veterinary Clinical Epidemiology. 3rd ed. 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