{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:15Z","timestamp":1777930755216,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,8,4]],"date-time":"2019-08-04T00:00:00Z","timestamp":1564876800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100019174","name":"Australian Companion Animal Health Foundation","doi-asserted-by":"crossref","award":["015 2015"],"award-info":[{"award-number":["015 2015"]}],"id":[{"id":"10.13039\/501100019174","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:p>\n                    DNA amplification by PCR detects KIT exon 11 internal tandem duplications in canine mast cell tumors (MCTs). Tissue-specific inhibitors often contaminate DNA extracted from formalin-fixed, paraffin-embedded (FFPE) canine MCTs, blocking PCR amplification and, consequently, preventing mutation detection. We used a commercial kit to extract DNA from FFPE canine MCTs. Two independent PCR assays, each with one primer set, were used to amplify target genes ( HPRT and KIT) directly after FFPE DNA extraction. PCR amplification failed with at least one primer set in 153 of 280 samples (54.6%, 95% CI: 48.8\u201360.5%). One or 2 DNA washing steps were required to remove PCR inhibitors in 130 of 280 (46.4%) and 23 of 280 (8.2%) of these cases, respectively. DNA concentration and quality (A\n                    <jats:sub>260<\/jats:sub>\n                    \/A\n                    <jats:sub>280<\/jats:sub>\n                    and A\n                    <jats:sub>260<\/jats:sub>\n                    \/A\n                    <jats:sub>230<\/jats:sub>\n                    ) either pre- or post-washing were not associated with ability of the samples to be amplified by PCR using both HPRT and KIT primer sets. Low-grade and subcutaneous MCTs were less likely to amplify directly after DNA extraction and without any washing steps compared to high-grade MCTs using KIT gene primers.\n                  <\/jats:p>","DOI":"10.1177\/1040638719867743","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T00:44:50Z","timestamp":1564965890000},"page":"756-760","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["DNA purification increases PCR-amplifiable DNA extracted from formalin-fixed, paraffin-embedded canine mast cell tumors for routine\n                    <i>KIT<\/i>\n                    mutation detection"],"prefix":"10.1177","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2732-5369","authenticated-orcid":false,"given":"Vanessa S.","family":"Tamlin","sequence":"first","affiliation":[{"name":"School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia (Tamlin, Woolford, Peaston)"},{"name":"Gribbles Veterinary Pathology, Clayton, Victoria, Australia (Dobson)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth C.","family":"Dobson","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia (Tamlin, Woolford, Peaston)"},{"name":"Gribbles Veterinary Pathology, Clayton, Victoria, Australia (Dobson)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucy","family":"Woolford","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia (Tamlin, Woolford, Peaston)"},{"name":"Gribbles Veterinary Pathology, Clayton, Victoria, Australia (Dobson)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne E.","family":"Peaston","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia (Tamlin, Woolford, Peaston)"},{"name":"Gribbles Veterinary Pathology, Clayton, Victoria, Australia (Dobson)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,8,4]]},"reference":[{"key":"bibr1-1040638719867743","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1996"},{"key":"bibr2-1040638719867743","first-page":"159","volume":"28","author":"Bessetti J","year":"2007","journal-title":"J Microbiol Methods"},{"key":"bibr3-1040638719867743","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2012.00341.x"},{"key":"bibr4-1040638719867743","first-page":"55","volume":"8","author":"Dedhia P","year":"2007","journal-title":"Asian Pac J Cancer 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