{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:29Z","timestamp":1777930769214,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T00:00:00Z","timestamp":1584576000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:p>Avian coxiellosis is an emerging cause of morbidity and mortality among captive psittacines, and the utility of a rapid detection test using easily obtained samples is paramount in a clinical setting. New sequences were obtained from 3 genes: groEL, dnaK, and rpoB. We developed probe-hybridization quantitative PCR (qPCR) assays using groEL and dnaK genes. Samples, including splenic aspirates, liver aspirates, whole blood, and choanal, conjunctival, and cloacal swabs, were collected from 4\u2009psittacine species including 3 blue-and-gold macaws ( Ara ararauna), 2 scarlet-chested parrots ( Neophema splendida), 1 Timneh African grey parrot ( Psittacus timneh), and 1 yellow-naped Amazon parrot ( Amazona auropalliata). Retrospective review of postmortem findings from 3 of these psittacines included splenomegaly, hepatitis, and\/or transmission electron microscopy confirmation consistent with previous reports of avian coxiellosis. There was 100% agreement between these assays and consensus PCR with sequencing. A Wilcoxon rank-sum test found a strong correlation between groEL and dnaK cycle threshold values ( p &lt; 0.001), validating these assays for detection of this avian Coxiella sp.<\/jats:p>","DOI":"10.1177\/1040638720912221","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T05:00:32Z","timestamp":1584594032000},"page":"423-428","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Development and validation of 2 probe-hybridization quantitative PCR assays for rapid detection of a pathogenic\n                    <i>Coxiella<\/i>\n                    species in captive psittacines"],"prefix":"10.1177","volume":"32","author":[{"given":"Alison J.","family":"Flanders","sequence":"first","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Speer","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drury R.","family":"Reavill","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Roberts","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"April L.","family":"Childress","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvin","family":"Atlas","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5692-6134","authenticated-orcid":false,"given":"James F. X.","family":"Wellehan","sequence":"additional","affiliation":[{"name":"University of Florida College of Veterinary Medicine, Gainesville, FL (Flanders, Roberts, Childress, Wellehan)"},{"name":"The Medical Center for Birds, Oakley, CA (Speer)"},{"name":"Zoo\/Exotic Pathology Service, Carmichael, CA (Reavill)"},{"name":"College of Veterinary Medicine, Auburn University, Auburn, AL (Roberts)"},{"name":"Riverview Animal Clinic, Birmingham, AL (Atlas)"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,3,19]]},"reference":[{"key":"bibr1-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2015.10.012"},{"key":"bibr2-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1638\/2016-0187R.1"},{"key":"bibr3-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2014.04.003"},{"key":"bibr4-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004892"},{"key":"bibr5-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a121298"},{"key":"bibr6-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1647\/2016-224"},{"key":"bibr7-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2013.840534"},{"key":"bibr8-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"bibr9-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1007\/BF00214484"},{"key":"bibr10-1040638720912221","doi-asserted-by":"publisher","DOI":"10.2307\/1592679"},{"key":"bibr11-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00454-09"},{"key":"bibr12-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.734-740.2004"},{"key":"bibr13-1040638720912221","doi-asserted-by":"publisher","DOI":"10.2307\/1589294"},{"key":"bibr14-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1637\/8192-120707-Reg"},{"key":"bibr15-1040638720912221","doi-asserted-by":"publisher","DOI":"10.3354\/dao041115"},{"key":"bibr16-1040638720912221","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-34.2.310"},{"key":"bibr17-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1177\/0300985811409251"},{"key":"bibr18-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1354\/vp.45-2-247"},{"key":"bibr19-1040638720912221","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2004.06.002"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638720912221","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/1040638720912221","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/1040638720912221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:23:21Z","timestamp":1777681401000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/1040638720912221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,19]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1177\/1040638720912221"],"URL":"https:\/\/doi.org\/10.1177\/1040638720912221","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,19]]}}}