{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:32Z","timestamp":1777930772165,"version":"3.51.4"},"reference-count":20,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T00:00:00Z","timestamp":1586390400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality","award":["26116120"],"award-info":[{"award-number":["26116120"]}]},{"DOI":"10.13039\/100007540","name":"jiangsu agricultural science and technology innovation fund","doi-asserted-by":"publisher","award":["CX(19)3026"],"award-info":[{"award-number":["CX(19)3026"]}],"id":[{"id":"10.13039\/100007540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"priority academic program development of jiangsu higher education institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:p>An outbreak of inclusion body hepatitis caused by fowl adenovirus serotype 8 (FAdV-8) has caused significant economic losses in the poultry industry worldwide. However, a rapid serology test kit specific to FAdV-8 is not available to date. We developed a fiber-based ELISA using the purified GST-fiber of FAdV-8 as coating antigen to measure antibodies against FAdV-8. Specificity analysis showed that our ELISA could react with sera against FAdV-7, -8a, and -8b, but not with sera against the other pathogens tested. Moreover, detection of positive sera with our ELISA had 83% and 94% agreement with an indirect immunofluorescence assay (IFA) and a commercial ELISA from BioChek, respectively. Our ELISA was also effective in the detection of antibodies against FAdV-8 in sera from both experimentally infected and clinically vaccinated chickens. Our FAdV-8 fiber-based ELISA can be a valuable tool to specifically and sensitively detect antibodies against FAdV-7 and\/or -8 in infected or vaccinated chickens.<\/jats:p>","DOI":"10.1177\/1040638720913354","type":"journal-article","created":{"date-parts":[[2020,4,9]],"date-time":"2020-04-09T07:15:36Z","timestamp":1586416536000},"page":"444-449","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["An efficient fiber-based ELISA for detection of antibody against fowl adenovirus serotypes 7 and 8"],"prefix":"10.1177","volume":"32","author":[{"given":"Hao","family":"Lu","sequence":"first","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, College of Veterinary Medicine; and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education of China; and Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu, China (Lu, Wang, Shao, L. 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