{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:10:11Z","timestamp":1787019011622,"version":"build-2736575974"},"reference-count":47,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Swine Health Information Center","award":["17-201"],"award-info":[{"award-number":["17-201"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>We evaluated the detection of pseudorabies virus (PRV) antibodies in swine oral fluid. Oral fluid and serum samples were obtained from 40 pigs allocated to 4 treatment groups (10 pigs\/group): negative control (NC); wild-type PRV inoculation (PRV 3CR Ossabaw; hereafter PRV); PRV vaccination (Ingelvac Aujeszky MLV; Boehringer Ingelheim; hereafter MLV); and PRV vaccination followed by PRV inoculation at 21\u2009d post-vaccination (MLV-PRV). Using a serum PRV whole-virus indirect IgG ELISA (Idexx Laboratories) adapted to the oral fluid matrix, PRV antibody was detected in oral fluid samples from treatment groups PRV, MLV, and MLV-PRV in a pattern similar to serum. Vaccination alone produced a low oral fluid antibody response (groups MLV and MLV-PRV), but a strong anamnestic response was observed following challenge with wild-type virus (group PRV). Analyses of the oral fluid PRV indirect IgG ELISA results showed good binary diagnostic performance (area under ROC curve = 93%) and excellent assay repeatability (intra-class correlation coefficient = 99.3%). The demonstrable presence of PRV antibodies in swine oral fluids suggests the possible use of oral fluids in pseudorabies surveillance.<\/jats:p>","DOI":"10.1177\/1040638720924386","type":"journal-article","created":{"date-parts":[[2020,5,26]],"date-time":"2020-05-26T02:19:30Z","timestamp":1590459570000},"page":"535-541","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Detection of pseudorabies virus antibody in swine oral fluid using a serum whole-virus indirect ELISA"],"prefix":"10.1177","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3732-9147","authenticated-orcid":false,"given":"Ting-Yu","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Cheng, Henao-D\u00edaz, Poonsuk, Pi\u00f1eyro, Baum, Main, Zimmerman, Gim\u00e9nez-Lirola), Iowa State University, Ames, IA"},{"name":"Department of Statistics, College of Liberal Arts and Sciences (Ji, Wang), Iowa State University, Ames, IA"},{"name":"Agricultural Research Service, U.S. Department of Agriculture, Ames, IA (Buckley, Van Geelen, Lager)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1821-7641","authenticated-orcid":false,"given":"Alexandra","family":"Buckley","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Cheng, Henao-D\u00edaz, Poonsuk, Pi\u00f1eyro, Baum, Main, Zimmerman, Gim\u00e9nez-Lirola), Iowa State University, Ames, IA"},{"name":"Department of Statistics, College of Liberal Arts and Sciences (Ji, Wang), Iowa State University, Ames, IA"},{"name":"Agricultural Research Service, U.S. Department of Agriculture, Ames, IA (Buckley, Van Geelen, Lager)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Van 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Lager)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Baum","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Cheng, Henao-D\u00edaz, Poonsuk, Pi\u00f1eyro, Baum, Main, Zimmerman, Gim\u00e9nez-Lirola), Iowa State University, Ames, IA"},{"name":"Department of Statistics, College of Liberal Arts and Sciences (Ji, Wang), Iowa State University, Ames, IA"},{"name":"Agricultural Research Service, U.S. Department of Agriculture, Ames, IA (Buckley, Van Geelen, Lager)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine (Cheng, Henao-D\u00edaz, Poonsuk, Pi\u00f1eyro, Baum, Main, Zimmerman, Gim\u00e9nez-Lirola), Iowa State University, Ames, IA"},{"name":"Department of Statistics, College of 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