{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:42Z","timestamp":1777930782158,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009947","name":"Merck Sharp and Dohme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009947","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001054","name":"Meat and Livestock Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>\n                    Salmonella spp. are common causes of disease in intensive livestock production systems, and contamination of foodstuffs is of significant concern for public health. Therefore, the identification and quantification of Salmonella spp. is important for monitoring the level of fecal shedding or tissue colonization in infected animals and animal products. We developed and evaluated a quantitative PCR (qPCR) method on spiked sheep tissue and fecal samples for the detection and quantification of Salmonella spp. Without the use of a pre-enrichment step, the qPCR limit of detection (LOD) results for sheep fecal (4 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    \u20136 \u00d7 10\n                    <jats:sup>3<\/jats:sup>\n                    \u2009cfu\/g) and tissue (4 \u00d7 10\n                    <jats:sup>5<\/jats:sup>\n                    \u20134 \u00d7 10\n                    <jats:sup>3<\/jats:sup>\n                    \u2009cfu\/g) samples were not adequate for detection purposes. With the inclusion of a 6-h pre-enrichment step in buffered peptone water (BPW), the LOD was 9\u2009cfu\/g (2.57 \u00d7 10\n                    <jats:sup>1<\/jats:sup>\n                    \u2009copies\/g) in sheep feces, and 5.4\u2009cfu\/g (3.22\u2009copies\/g) sheep tissue. Comparison of the 6-h BPW qPCR method with a 24-h mannitol\u2013selenite\u2013cystine broth enrichment culture method using spiked samples revealed a sensitivity of 91% and 92%, respectively, and a specificity of 100% for both methods. The correlation was significant between the quantity (copies\/mL) of Salmonella spp. in BPW at 6\u2009h and at 0\u2009h, allowing semiquantitative analysis. Our results demonstrate that, following inclusion of a 6-h pre-enrichment step in BPW, qPCR is semiquantitative with improved LODs of Salmonella spp. in sheep fecal and tissue samples.\n                  <\/jats:p>","DOI":"10.1177\/1040638720952359","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T07:24:18Z","timestamp":1598599458000},"page":"835-843","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Development of a qPCR for the detection and quantification of\n                    <i>Salmonella<\/i>\n                    spp. in sheep feces and tissues"],"prefix":"10.1177","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6122-8645","authenticated-orcid":false,"given":"Alysia M.","family":"Parker","sequence":"first","affiliation":[{"name":"Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia L.","family":"Mohler","sequence":"additional","affiliation":[{"name":"Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison A.","family":"Gunn","sequence":"additional","affiliation":[{"name":"Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John K.","family":"House","sequence":"additional","affiliation":[{"name":"Faculty of Science, Sydney School of Veterinary Science, The University of Sydney, Camden, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,8,28]]},"reference":[{"issue":"9","key":"bibr1-1040638720952359","first-page":"117","volume":"17","author":"AOAC","year":"2005","journal-title":"AOAC Int"},{"key":"bibr2-1040638720952359","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12359"},{"key":"bibr3-1040638720952359","doi-asserted-by":"publisher","DOI":"10.1177\/104063870501700603"},{"key":"bibr4-1040638720952359","first-page":"23","volume":"69","author":"Chen Z","year":"2019","journal-title":"Lett Appl 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