{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:46Z","timestamp":1777930786895,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T00:00:00Z","timestamp":1603238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Proyecto FIC 15-8","award":["c\u00f3digo BIP 30421496"],"award-info":[{"award-number":["c\u00f3digo BIP 30421496"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Clinical manifestations of leptospirosis are diverse and very similar to other febrile diseases, hence early and accurate detection of subclinical infections is a key element in disease control. We evaluated immunomagnetic separation (IMS) capture technology coupled with a standard quantitative PCR (qPCR) system for the detection of pathogenic Leptospira in urine samples from 803 cows from dairy herds with a history of clinical cases of leptospirosis. The urine samples were first processed in a purification step, then subdivided into 2 subsamples, one that continued to DNA extraction and direct qPCR, and one that was pretreated by IMS before continuing to DNA extraction and qPCR. Overall, 133 of 803 (16.6%) samples were IMS-qPCR positive, whereas only 92 of 803 (11.5%) were positive when using direct qPCR. Statistically significant differences were observed between the mean estimated Leptospira load between the IMS-qPCR and the direct qPCR positive urine samples. The IMS-qPCR technology revealed a larger number of positive results and higher bacterial loads than direct qPCR. This difference is most likely the result of the high antigen-binding capacity and capture efficiency of the IMS system. The use of polyclonal antibodies produced by the inoculation of 3 synthetic peptides, which make up the extracellular regions of the LipL32 protein, provided a high detection capacity to the IMS-qPCR technique, resulting in performance superior to direct qPCR.<\/jats:p>","DOI":"10.1177\/1040638720966299","type":"journal-article","created":{"date-parts":[[2020,10,21]],"date-time":"2020-10-21T08:44:52Z","timestamp":1603269892000},"page":"52-58","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Analytical evaluation of an immunomagnetic separation PCR assay to detect pathogenic\n                    <i>Leptospira<\/i>\n                    in cattle urine samples obtained under field conditions"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-9265","authenticated-orcid":false,"given":"Camilo","family":"Tomckowiack","sequence":"first","affiliation":[{"name":"Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Henriquez","sequence":"additional","affiliation":[{"name":"Instituto de Farmacolog\u00eda y Morfofisiolog\u00eda, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Ramirez-Reveco","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardita","family":"Collado","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Herzberg","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Cl\u00ednicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo","family":"Folch","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Facultad de Medicina, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2144-7982","authenticated-orcid":false,"given":"Miguel","family":"Salgado","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Preventiva Veterinaria, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2020,10,21]]},"reference":[{"key":"bibr1-1040638720966299","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"bibr2-1040638720966299","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"bibr3-1040638720966299","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-3-251"},{"key":"bibr4-1040638720966299","unstructured":"Faine S, et al.\n                      Leptospira\n                      and Leptospirosis. 2nd ed. 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