{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:28:30Z","timestamp":1791872910959,"version":"4.3.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100011003","name":"Instituto Nacional de Investigaci\u00f3n Agropecuaria","doi-asserted-by":"publisher","award":["Determination of the current situation of Rhipicep"],"award-info":[{"award-number":["Determination of the current situation of Rhipicep"]}],"id":[{"id":"10.13039\/501100011003","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Detection of bovine Babesia spp. and Anaplasma marginale is based on the reading of Giemsa-stained blood or organ smears, which can have low sensitivity. Our aim was to improve the detection of bovine Babesia spp. and A. marginale by validating a multiplex PCR (mPCR). We used 466 samples of blood and\/or organs of animals with signs and presumptive autopsy findings of babesiosis or anaplasmosis. The primers in our mPCR amplified the rap-1a gene region of Babesia bovis and B. bigemina, and the msp-5 region of A. marginale. We used a Bayesian model with a non-informative priori distribution for the prevalence estimate and informative priori distribution for estimation of sensitivity and specificity. The sensitivity and specificity for smear detection of Babesia spp. were 68.6% and 99.1%, and for A. marginale 85.6% and 98.8%, respectively. Sensitivity and specificity for mPCR detection for Babesia spp. were 94.2% and 97.1%, and for A. marginale 95.2% and 92.7%, respectively. Our mPCR had good accuracy in detecting Babesia spp. and A. marginale, and would be a reliable test for veterinarians to choose the correct treatment for each agent.<\/jats:p>","DOI":"10.1177\/1040638720975742","type":"journal-article","created":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T08:03:35Z","timestamp":1606723415000},"page":"73-79","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":24,"title":["Validation of a multiplex PCR assay to detect\n                    <i>Babesia<\/i>\n                    spp. and\n                    <i>Anaplasma marginale<\/i>\n                    in cattle in Uruguay in the absence of a gold standard test"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9878-2930","authenticated-orcid":false,"given":"Pablo","family":"Parodi","sequence":"first","affiliation":[{"name":"Instituto Nacional de Investigaci\u00f3n Agropecuaria, Plataforma de Salud Animal, Estaci\u00f3n Experimental INIA, Tacuaremb\u00f3, Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis G.","family":"Corbellini","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiolog\u00eda Veterin\u00e1ria, Facultad de Veterin\u00e1ria, Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa B.","family":"Leotti","sequence":"additional","affiliation":[{"name":"Departamento de Estat\u00edstica, Instituto de Matem\u00e1tica e Estat\u00edstica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Rivero","sequence":"additional","affiliation":[{"name":"Laboratorio Regional Noroeste \u201cMiguel C. 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