{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:39:56Z","timestamp":1777930796987,"version":"3.51.4"},"reference-count":5,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"HORIBA ABX SAS"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:p>Dirofilaria immitis causes life-threatening heart disease in dogs, thus screening of dog populations is important. Lens-free technology (LFT) is a low-cost imaging technique based on light diffraction that allows computerized recognition of small objects in holographic images. We evaluated an algorithm capable of recognizing microfilariae in canine whole blood using the LFT. We examined 3 groups of 10 EDTA blood specimens, from dogs with microfilaremia (group A), healthy dogs (B), and dogs with hematologic modifications other than microfilaremia (C). The LFT analyzer photographed repeated series of 5 images of all samples. The algorithm declared a sample positive if a microfilaria was detected on \u22651, \u22652, or \u22653 of the 5 images of a series. Microfilariae were detected visually in the images in 9 of 10 cases in group A; no microfilariae were seen in the images from groups B and C. Of the 30 cases, there were 14, 4, and only 3 false-positives with the 1 of 5, 2 of 5, and 3 of 5 image cutoffs, respectively. There were no false-negatives, regardless of cutoff. LFT seems useful for detecting microfilaria and could have application in clinical pathology.<\/jats:p>","DOI":"10.1177\/10406387211001092","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T07:13:45Z","timestamp":1616051625000},"page":"572-576","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of circulating microfilariae in canine EDTA blood using lens-free technology: preliminary results"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0696-0101","authenticated-orcid":false,"given":"Typhaine","family":"Lavabre","sequence":"first","affiliation":[{"name":"D\u00e9partement des Sciences Cliniques des animaux de compagnie et de sport, \u00c9cole nationale v\u00e9t\u00e9rinaire de Toulouse, Universit\u00e9 de Toulouse, INSERM, ENVT, UPS, Toulouse, France"},{"name":"CREFRE, \u00c9cole nationale v\u00e9t\u00e9rinaire de Toulouse, Universit\u00e9 de Toulouse, INSERM, ENVT, UPS, Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4039-2398","authenticated-orcid":false,"given":"Zoe S.","family":"Polizopoulou","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damien","family":"Is\u00e8be","sequence":"additional","affiliation":[{"name":"HORIBA, Montpellier, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Cioni","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Grenoble Alpes, CEA, LETI, Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e9ronique","family":"Rebuffel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Grenoble Alpes, CEA, LETI, Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Blandin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Grenoble Alpes, CEA, LETI, Grenoble, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-8131","authenticated-orcid":false,"given":"Nathalie","family":"Bourg\u00e8s-Abella","sequence":"additional","affiliation":[{"name":"CREFRE, \u00c9cole nationale v\u00e9t\u00e9rinaire de Toulouse, Universit\u00e9 de Toulouse, INSERM, ENVT, UPS, Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Trumel","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Cliniques des animaux de compagnie et de sport, \u00c9cole nationale v\u00e9t\u00e9rinaire de Toulouse, Universit\u00e9 de Toulouse, INSERM, ENVT, UPS, Toulouse, France"},{"name":"CREFRE, \u00c9cole nationale v\u00e9t\u00e9rinaire de Toulouse, 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