{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:13:04Z","timestamp":1790259184746,"version":"4.1.0"},"reference-count":20,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T00:00:00Z","timestamp":1618444800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"the Key Research & Development (R&D) Plan in Yangzhou City","award":["YZ2020052"],"award-info":[{"award-number":["YZ2020052"]}]},{"DOI":"10.13039\/100007540","name":"Jiangsu Agricultural Science and Technology Innovation Fund","doi-asserted-by":"publisher","award":["CX(19)3026"],"award-info":[{"award-number":["CX(19)3026"]}],"id":[{"id":"10.13039\/100007540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>Fowl adenovirus serotype 4 (FAdV4), the causative agent of hepatitis-hydropericardium syndrome (HPS), has caused major economic losses to the poultry industry worldwide. Although inactivated vaccines have been deployed widely against FAdV4, a DIVA (differentiating infected from vaccinated animals) test specific for FAdV4 has not been available. We synthesized an immunogenic peptide, corresponding to regions 66\u201388 aa of the 22K nonstructural protein of FAdV4, and used the peptide as coating antigen to develop an indirect ELISA for a DIVA test specific to FAdV4. Specificity analysis showed that the ELISA only reacted with sera against FAdV4, and not with sera against other pathogens tested. Moreover, the ELISA could effectively differentiate FAdV4\u2013infected chickens from vaccinated chickens. In a test of sera from experimentally infected chickens, the ELISA had 95% and 85% concordance with an indirect immunofluorescence assay (indirect IFA) and a commercial ELISA, respectively, and the concordance was 80.5% between the ELISA and the indirect IFA in detecting clinical infection samples. Our peptide-based ELISA provides an efficient DIVA test for FAdV4 in clinical samples.<\/jats:p>","DOI":"10.1177\/10406387211005749","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T18:13:25Z","timestamp":1618510405000},"page":"762-766","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["An efficient peptide-based ELISA for differentiating fowl adenovirus 4\u2013infected chickens from vaccinated chickens"],"prefix":"10.1177","volume":"33","author":[{"given":"Songhua","family":"Xie","sequence":"first","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuping","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sinopharm Yangzhou VAC Biological Engineering, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikang","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuofan","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Wan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Shao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijian","family":"Qin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3940-1357","authenticated-orcid":false,"given":"Jianqiang","family":"Ye","sequence":"additional","affiliation":[{"name":"Key Laboratory of Jiangsu Preventive Veterinary Medicine, Key Laboratory for Avian Preventive Medicine, Ministry of Education, and Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education, and Institutes of Agricultural Science and Technology Development, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"key":"bibr1-10406387211005749","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01503"},{"key":"bibr2-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1969-7"},{"key":"bibr3-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2017.1372561"},{"key":"bibr4-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2018.03.002"},{"key":"bibr5-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.01.002"},{"key":"bibr6-10406387211005749","doi-asserted-by":"crossref","unstructured":"Harrach B, et al. Family\n                      Adenoviridae\n                      . In: King AMQ, et al., eds. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier, 2012:125\u2013141.","DOI":"10.1016\/B978-0-12-384684-6.00009-4"},{"key":"bibr7-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1637\/11758-100917-Reg.1"},{"key":"bibr8-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.09.025"},{"key":"bibr9-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0443"},{"key":"bibr10-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1007\/s13337-013-0183-7"},{"key":"bibr11-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-015-2635-4"},{"key":"bibr12-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00123-06"},{"key":"bibr13-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2017.1385724"},{"key":"bibr14-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.12.056"},{"key":"bibr15-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2019.1566595"},{"key":"bibr16-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1987-5"},{"key":"bibr17-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01463-12"},{"key":"bibr18-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2013.829553"},{"key":"bibr19-10406387211005749","volume":"5","author":"Yanke L","year":"2016","journal-title":"Emerg Microbes Infect"},{"key":"bibr20-10406387211005749","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2016.50"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387211005749","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387211005749","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387211005749","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:24:18Z","timestamp":1777681458000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387211005749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,15]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1177\/10406387211005749"],"URL":"https:\/\/doi.org\/10.1177\/10406387211005749","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,15]]}}}