{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T08:10:03Z","timestamp":1782979803162,"version":"3.54.5"},"reference-count":20,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100009068","name":"universit\u00e4t bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:p>Digital dermatitis (DD) is a major infectious foot disease of cattle worldwide. Some DD stages are associated with lameness, and the disease has significant economic and animal welfare consequences. The pathogenesis of the disease is not yet fully understood, but Treponema spp. have been associated consistently with clinical cases. Isolation of these fastidious bacteria is difficult and cumbersome. We describe an improved method enabling the culturing of the 3 Treponema spp. ( T. pedis, T. phagedenis, and T. medium) from bovine foot specimens derived from DD lesions, using a combination of membrane filtering and subsequent growth on selective agar media. The entire procedure from sampling to verification of individual Treponema spp. takes up to 24\u2009d. In addition, we established a MALDI-TOF MS\u2013based identification method to be applied for confirmation of the different Treponema spp. This scheme provides an unambiguous, simple, and straightforward identification procedure for DD-associated Treponema spp.<\/jats:p>","DOI":"10.1177\/10406387211008511","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T07:00:21Z","timestamp":1617951621000},"page":"801-805","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["A filter-assisted culture method for isolation of\n                    <i>Treponema<\/i>\n                    spp. from bovine digital dermatitis and their identification by MALDI-TOF MS"],"prefix":"10.1177","volume":"33","author":[{"given":"Isabelle","family":"Brodard","sequence":"first","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maher","family":"Alsaaod","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Gurtner","sequence":"additional","affiliation":[{"name":"Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joerg","family":"Jores","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Steiner","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1575-5225","authenticated-orcid":false,"given":"Peter","family":"Kuhnert","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"bibr1-10406387211008511","doi-asserted-by":"publisher","DOI":"10.17236\/sat00201"},{"key":"bibr2-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178349"},{"key":"bibr3-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0605-z"},{"key":"bibr4-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.06.048"},{"key":"bibr5-10406387211008511","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2009-2721"},{"key":"bibr6-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-2-196"},{"key":"bibr7-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00025-16"},{"key":"bibr8-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1136\/vr.140.24.620"},{"key":"bibr9-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2010.03.001"},{"key":"bibr10-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1136\/vr.c5487"},{"key":"bibr11-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2007.12.019"},{"key":"bibr12-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01914-08"},{"key":"bibr13-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.10.028"},{"key":"bibr14-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.03.016"},{"key":"bibr15-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.004027"},{"key":"bibr16-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1002\/9781118960608.gbm01249"},{"key":"bibr17-10406387211008511","unstructured":"Rau J, et al. MALDI-UP\u2014an internet platform for the exchange of MALDI-TOF mass spectra. Aspects Food Control Animal Health 2016;1:1\u201317. https:\/\/ejournal.cvuas.de\/issue201601.asp"},{"key":"bibr18-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.05.023"},{"key":"bibr19-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2522-2529.2003"},{"key":"bibr20-10406387211008511","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00114-X"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387211008511","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387211008511","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387211008511","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:24:21Z","timestamp":1777681461000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387211008511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,9]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,7]]}},"alternative-id":["10.1177\/10406387211008511"],"URL":"https:\/\/doi.org\/10.1177\/10406387211008511","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,9]]}}}