{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:40:12Z","timestamp":1777930812123,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia da Cadeia Produtiva do Leite","award":["465725\/ 2014\u20137"],"award-info":[{"award-number":["465725\/ 2014\u20137"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>\n                    We describe the molecular analysis of a wild-type field strain of bovine viral diarrhea virus (BVDV) identified in a mummified fetus from a small Brazilian dairy cattle herd. Nucleic acids extracted from samples of the lung, liver, heart, spleen, and kidney were tested by PCR assays for bovine alphaherpesvirus 1, Neospora caninum, Leptospira spp., Histophilus somni, and Brucella abortus, a nested PCR assay for Mycoplasma bovigenitalium and Ureaplasma diversum, and a RT-PCR assay for BVDV. Amplicons were only obtained in the RT-PCR assay for the partial amplification of the BVDV 5\u2032UTR (288\u2009bp) in kidney and spleen samples and the N\n                    <jats:sup>pro<\/jats:sup>\n                    (438\u2009bp) gene in the kidney sample. Nucleotide sequencing of the amplified products and phylogenetic analyses based on the 2 BVDV genomic regions enabled the BVDV strain to be classified as subgenotype 1a.\n                  <\/jats:p>","DOI":"10.1177\/10406387211022012","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T02:29:51Z","timestamp":1622687391000},"page":"966-968","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Bovine viral diarrhea virus subgenotype 1a in a mummified fetus from a Brazilian dairy cattle herd"],"prefix":"10.1177","volume":"33","author":[{"given":"Juliana T. T.","family":"Fritzen","sequence":"first","affiliation":[{"name":"Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur B.","family":"Morettin","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elis","family":"Lorenzetti","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Brazil"},{"name":"Multi-User Animal Health Laboratory, Molecular Biology Unit, Universidade Estadual de Londrina, Londrina, Brazil"},{"name":"Post Graduate Program in Animal Health and Production, Universidade Pit\u00e1goras Unopar, Arapongas, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alice F.","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Brazil"},{"name":"Multi-User Animal Health Laboratory, Molecular Biology Unit, Universidade Estadual de Londrina, Londrina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amauri A.","family":"Alfieri","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Virology, Universidade Estadual de Londrina, Londrina, Brazil"},{"name":"Department of Veterinary Preventive Medicine, and National Institute of Science and Technology for Dairy Production Chain (INCT\u2013LEITE), Universidade Estadual de Londrina, Londrina, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"bibr1-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-006-4349-9"},{"key":"bibr2-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"bibr3-10406387211022012","doi-asserted-by":"publisher","DOI":"10.20506\/rst.9.1.491"},{"key":"bibr4-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-736X2006000400005"},{"key":"bibr5-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2019.108565"},{"key":"bibr6-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00274-1"},{"key":"bibr7-10406387211022012","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2003.11.006"},{"key":"bibr8-10406387211022012","unstructured":"International Committee on Taxonomy of Viruses. 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