{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:40:17Z","timestamp":1785408017949,"version":"3.56.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>Atypical porcine pestivirus (APPV) is a cause of congenital tremors (CTs) in piglets and has been found in swine populations around the globe. Although systemic distribution of the virus has been reported, there is limited information regarding viral localization at the cellular level and distribution at the tissue level. We collected multiple tissues from 2-d-old piglets ( n\u2009=\u200936) born to sows inoculated at 45 or 62\u2009d of gestation with APPV via 3 simultaneous routes: intravenous, intranasal, and directly in amniotic vesicles. In addition, 2 boars from APPV-inoculated sows with CT were raised and euthanized when 11\u2009mo old. In situ hybridization performed on tissue samples from piglets demonstrated a broad and systemic distribution of viral RNA including endothelial cells, fibroblasts, and smooth muscle. Labeling in tissues was more pronounced in piglet tissues compared to boars, with the notable exception of diffuse labeling of the cerebellum in boars. Presence of APPV in boar tissues well after resolution of clinical signs suggests persistence of APPV similar to other pestiviruses.<\/jats:p>","DOI":"10.1177\/10406387211022683","type":"journal-article","created":{"date-parts":[[2021,6,3]],"date-time":"2021-06-03T02:32:14Z","timestamp":1622687534000},"page":"952-955","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Distribution and persistence of atypical porcine pestivirus in experimentally inoculated pigs"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5892-7042","authenticated-orcid":false,"given":"Alexandra C.","family":"Buckley","sequence":"first","affiliation":[{"name":"Virus and Prion Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shollie M.","family":"Falkenberg","sequence":"additional","affiliation":[{"name":"Ruminant Diseases and Immunology Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5902-3333","authenticated-orcid":false,"given":"Mitchell V.","family":"Palmer","sequence":"additional","affiliation":[{"name":"Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agriculture Research Service, U.S. Department of Agriculture, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo H.","family":"Arruda","sequence":"additional","affiliation":[{"name":"VRI\/AMVC Audubon Manning Veterinary Clinic, Ames, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drew R.","family":"Magstadt","sequence":"additional","affiliation":[{"name":"Veterinary Diagnostic and Production Animal 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