{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:54:50Z","timestamp":1780548890782,"version":"3.54.1"},"reference-count":20,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["CEITEC 2020 (LQ1601)"],"award-info":[{"award-number":["CEITEC 2020 (LQ1601)"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:p>We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306\u2009bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4\u2009pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.<\/jats:p>","DOI":"10.1177\/10406387211032927","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T08:32:55Z","timestamp":1632299575000},"page":"1188-1192","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Species-specific PCR assay for the detection of\n                    <i>Babesia odocoilei<\/i>"],"prefix":"10.1177","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6663-3295","authenticated-orcid":false,"given":"Hilary J.","family":"Burgess","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betty P.","family":"Lockerbie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisanework E.","family":"Ayalew","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Dibernardo","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krist\u00fdna","family":"Hrazdilov\u00e1","sequence":"additional","affiliation":[{"name":"CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Modry","sequence":"additional","affiliation":[{"name":"CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic"},{"name":"Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic"},{"name":"Biology Centre, Institute of Parasitology, Czech Academy of Sciences, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trent K.","family":"Bollinger","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Canadian Wildlife Health Cooperative, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,9,22]]},"reference":[{"key":"bibr1-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.05.057"},{"key":"bibr2-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.63.111"},{"key":"bibr3-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1638\/2008-0011.1"},{"key":"bibr4-10406387211032927","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-35.3.458"},{"key":"bibr5-10406387211032927","doi-asserted-by":"publisher","DOI":"10.2307\/3282486"},{"key":"bibr6-10406387211032927","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-30.3.460"},{"key":"bibr7-10406387211032927","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-36.3.518"},{"key":"bibr8-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.11.052"},{"key":"bibr9-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.104060"},{"key":"bibr10-10406387211032927","doi-asserted-by":"publisher","DOI":"10.1177\/1040638714535600"},{"key":"bibr11-10406387211032927","unstructured":"Maniatis T, et al. 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