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We surveyed commercial pig herds in Brazil for viral and bacterial respiratory coinfections that could complicate EP. Over a 2-y period (2015\u20132016), we found that 854 of 2,206 pigs (38.7%) were positive for M. hyopneumoniae in herds from various production systems in 3 Brazilian regions (Central-West, Southeast, South). We collected samples of 321 lungs positive for M. hyopneumoniae from 169 farms to also screen for Pasteurella multocida, Actinobacillus pleuropneumoniae, Glaesserella parasuis, influenza A virus (IAV), and porcine circovirus 2 (PCV2) by real-time PCR. The prevalence of pathogens found in addition to M. hyopneumoniae varied: P. multocida (141; 43.9%), G. parasuis (71; 22.1%), PCV2 (50; 15.6%), IAV (23; 7.2%), and A. pleuropneumoniae (18; 5.6%). G. parasuis was more frequent in farrowing or nursery herds (48.7%) than in breeding and fattening herds (10% and 18.6%, respectively; p\u2009&lt;\u20090.01); A. pleuropneumoniae was found only in herds on farrow-to-finish and fattening farms.<\/jats:p>","DOI":"10.1177\/10406387211069552","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T04:14:33Z","timestamp":1642392873000},"page":"310-313","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular detection of respiratory coinfections in pig herds with enzootic pneumonia: a survey in Brazil"],"prefix":"10.1177","volume":"34","author":[{"given":"Eder","family":"Balestrin","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Diagnostic, Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas M.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostic, Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas M.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Diagnostic, Universidade Luterana do Brasil (ULBRA), Canoas, Rio Grande do Sul, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 S. 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