{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:40:41Z","timestamp":1777930841487,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100006507","name":"funda\u00e7\u00e3o oswaldo cruz","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006507","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus Metastrongylus, whose eggs are similar to other Strongylida through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of Metastrongylus infecting pigs in close contact with humans in impoverished rural communities in the state of Piau\u00ed, in northeastern Brazil. Fecal samples ( n\u2009=\u200978) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 ( cox1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with Metastrongylus. Of the 37 samples submitted to PCR, 10 were identified as M. salmi. We found 3 M. salmi haplotypes, including 2 new and 1 described previously in Europe. Overall, M. salmi demonstrated lower intraspecific genetic diversity: diversity index (H)\u2009\u00b1\u2009SD\u2009=\u20090.318\u2009\u00b1\u20090.164, n\u2009=\u200912, compared with published M. pudendotectus sequences (1.000\u2009\u00b1\u20090.272, n\u2009=\u20093). To our knowledge, M. salmi DNA sequences have not been published previously from pigs in South America.<\/jats:p>","DOI":"10.1177\/10406387221092143","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:56:06Z","timestamp":1653440166000},"page":"689-692","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular detection of\n                    <i>Metastrongylus salmi<\/i>\n                    eggs from pigs in low-resource communities in the state of Piau\u00ed, northeastern Brazil"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6509-2327","authenticated-orcid":false,"given":"Polyanna A. A.","family":"Bacelar","sequence":"first","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"},{"name":"Oswaldo Cruz Foundation Regional Office\u2013Piau\u00ed, Teresina, Piau\u00ed, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren H.","family":"Jaeger","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Juiz de Fora Federal University, Juiz de Fora, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deiviane A.","family":"Calegar","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica P.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"},{"name":"Oswaldo Cruz Foundation Regional Office\u2013Piau\u00ed, Teresina, Piau\u00ed, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Coronato-Nunes","sequence":"additional","affiliation":[{"name":"Arthur S\u00e1 Earp Neto University Center\/Petropolis Faculty of Medicine (UNIFASE\/FMP), Petr\u00f3polis, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elis R. C.","family":"Reis","sequence":"additional","affiliation":[{"name":"Municipal Health Department of Nossa Senhora de Nazar\u00e9, Nossa Senhora de Nazar\u00e9, Piau\u00ed, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio N.","family":"B\u00f3ia","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Parasitology of Wild Reservoirs Mammals, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerla J. L.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"},{"name":"Oswaldo Cruz Foundation Regional Office\u2013Piau\u00ed, Teresina, Piau\u00ed, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe A.","family":"Carvalho-Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,5,24]]},"reference":[{"key":"bibr1-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026036"},{"key":"bibr2-10406387221092143","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0247"},{"key":"bibr3-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182001007223"},{"key":"bibr4-10406387221092143","first-page":"13","volume":"27","author":"da Paix\u00e3o Sev\u00e1 A","year":"2018","journal-title":"Rev Bras Parasitol Vet"},{"key":"bibr5-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2010.02847.x"},{"key":"bibr6-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-4001-y"},{"key":"bibr7-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp883"},{"key":"bibr8-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06899-7"},{"key":"bibr9-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/8242486"},{"key":"bibr10-10406387221092143","doi-asserted-by":"publisher","DOI":"10.2307\/3284232"},{"key":"bibr11-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.04.036"},{"key":"bibr12-10406387221092143","doi-asserted-by":"publisher","DOI":"10.32406\/v3n1\/2020\/122-129\/agrariacad"},{"key":"bibr13-10406387221092143","first-page":"9","volume":"23","author":"Messing J.","year":"1993","journal-title":"Methods Mol Biol"},{"key":"bibr14-10406387221092143","first-page":"227","volume":"8","author":"Milosev BI.","year":"1963","journal-title":"Microbiol Parazitol Epidemiol (Bucur)"},{"key":"bibr15-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1590\/s1678-9946201860024"},{"key":"bibr16-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-010-9732-x"},{"key":"bibr17-10406387221092143","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-24.1.105"},{"key":"bibr18-10406387221092143","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1111\/1755-0998.12082","volume":"13","author":"Prosser SWJ","year":"2013","journal-title":"Mol Ecol Resour"},{"key":"bibr19-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5296-7"},{"key":"bibr20-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msx248"},{"key":"bibr21-10406387221092143","doi-asserted-by":"publisher","DOI":"10.3961\/jpmph.17.097"},{"key":"bibr22-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1079\/JOH2003168"},{"key":"bibr23-10406387221092143","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X21000353"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221092143","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387221092143","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221092143","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:25:38Z","timestamp":1777681538000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387221092143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,24]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1177\/10406387221092143"],"URL":"https:\/\/doi.org\/10.1177\/10406387221092143","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,24]]}}}