{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:12:29Z","timestamp":1790298749367,"version":"4.1.0"},"reference-count":18,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2022,7,21]],"date-time":"2022-07-21T00:00:00Z","timestamp":1658361600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100006492","name":"division of intramural research, national institute of allergy and infectious diseases","doi-asserted-by":"publisher","award":["U19AI110820, AI114009-02"],"award-info":[{"award-number":["U19AI110820, AI114009-02"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for Borrelia burgdorferi in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the B. burgdorferi flagellin gene was negative. However, we enriched B. burgdorferi DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified B. burgdorferi in the CSF of the horse, confirming a diagnosis of neuroborreliosis.<\/jats:p>","DOI":"10.1177\/10406387221112617","type":"journal-article","created":{"date-parts":[[2022,7,22]],"date-time":"2022-07-22T01:25:42Z","timestamp":1658453142000},"page":"909-912","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Genomic hybrid capture assay to detect\n                    <i>Borrelia burgdorferi<\/i>\n                    : an application to diagnose neuroborreliosis in horses"],"prefix":"10.1177","volume":"34","author":[{"given":"Thomas J.","family":"Divers","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Cornell University, Ithaca, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel F.","family":"Mongodin","sequence":"additional","affiliation":[{"name":"Institute of Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher B.","family":"Miller","sequence":"additional","affiliation":[{"name":"Miller and Associates Equine Practice, Brewster, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney L.","family":"Belgrave","sequence":"additional","affiliation":[{"name":"Mid-Atlantic Equine Hospital, Ringoes, NJ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel B.","family":"Gardner","sequence":"additional","affiliation":[{"name":"B.W. 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