{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:40:51Z","timestamp":1777930851076,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,8,2]],"date-time":"2022-08-02T00:00:00Z","timestamp":1659398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>Clonality assays based on antigen receptors are used as adjunct examinations in the diagnosis of lymphoproliferative diseases. We investigated the usefulness of the T-cell receptor beta ( TRB) and T-cell receptor delta ( TRD) loci in clonality assays for high-grade gastrointestinal (GI) lymphoma in dogs. For TRB, we used primers reported previously; for TRD, we designed primers for each of the V and J genes based on genomic sequences. Genomic DNA was extracted from 39 formalin-fixed, paraffin-embedded sections of high-grade GI lymphoma diagnosed histologically. The sensitivity of TRB and TRD primers for GI lymphoma was 41.0% and 38.5%, respectively, which was lower than the 82.1% sensitivity of T-cell receptor gamma ( TRG) primers However, some cases that could not be detected using TRG primers had clonality with either TRB or TRD primers. We found the TRG locus to be more suitable as a first choice for the assay of canine lymphoma clonality than the TRB and TRD loci. However, the detection rate of T-cell clonality may be enhanced using TRB and TRD primers for lymphoma cases not detected using TRG primers.<\/jats:p>","DOI":"10.1177\/10406387221116285","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T01:45:11Z","timestamp":1659491111000},"page":"972-976","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["A clonality analysis based on T-cell receptor beta and delta loci for high-grade gastrointestinal lymphoma in dogs"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5500-7811","authenticated-orcid":false,"given":"Masamine","family":"Takanosu","sequence":"first","affiliation":[{"name":"Nasunogahara Animal Clinic, Ohtawara, Tochigi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Kagawa","sequence":"additional","affiliation":[{"name":"North Lab, Sapporo, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,8,2]]},"reference":[{"key":"bibr1-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1354\/vp.40-1-32"},{"key":"bibr2-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-269-8_4"},{"key":"bibr3-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20042524"},{"key":"bibr4-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1159\/000086659"},{"key":"bibr5-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.71.555"},{"key":"bibr6-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7477"},{"key":"bibr7-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.12.019"},{"key":"bibr8-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1177\/0300985812451625"},{"key":"bibr9-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815626576"},{"key":"bibr10-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810389479"},{"key":"bibr11-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.246"},{"key":"bibr12-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-2154-8"},{"key":"bibr13-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2012.03.010"},{"key":"bibr14-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.08.011"},{"key":"bibr15-10406387221116285","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00821"},{"key":"bibr16-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16288"},{"key":"bibr17-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12494"},{"key":"bibr18-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1177\/1040638715600197"},{"key":"bibr19-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2018.09.010"},{"key":"bibr20-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2019.109903"},{"key":"bibr21-10406387221116285","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221116285","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387221116285","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221116285","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:25:56Z","timestamp":1777681556000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387221116285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,2]]},"references-count":21,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1177\/10406387221116285"],"URL":"https:\/\/doi.org\/10.1177\/10406387221116285","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,2]]}}}