{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:40:53Z","timestamp":1777930853391,"version":"3.51.4"},"reference-count":10,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>We made 2 Z-based in situ hybridization (ISH) probes for the detection of rabbit hemorrhagic disease virus 2 (RHDV2; Lagovirus GI.2) nucleic acid in formalin-fixed, paraffin-embedded tissues from European rabbits ( Oryctolagus cuniculus) that had died during an outbreak of RHD in Washington, USA. One probe system was made for detection of negative-sense RNA (i.e., the replicative intermediate RNA for the virus), and the other probe system was constructed for detection of genomic and mRNA of the virus (viral mRNA). Tissue sets were tested separately, and the viral mRNA probe system highlighted much broader tissue distribution than that of the replicative intermediate RNA probe system. The latter was limited to liver, lung, kidney, spleen, myocardium, and occasional endothelial staining, whereas signal for the viral mRNA was seen in many more tissues. The difference in distribution suggests that innate phagocytic activity of various cell types may cause overestimation of viral replication sites when utilizing ISH of single-stranded, positive-sense viruses.<\/jats:p>","DOI":"10.1177\/10406387221126999","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T01:50:00Z","timestamp":1664416200000},"page":"1023-1026","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Viral replication site distribution for rabbit hemorrhagic disease virus 2 in formalin-fixed, paraffin-embedded tissues via in situ hybridization"],"prefix":"10.1177","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7989-3491","authenticated-orcid":false,"given":"Alicia D.","family":"O\u2019Toole","sequence":"first","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6229-4820","authenticated-orcid":false,"given":"Laura B. A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology & Pathology, Washington State University, Pullman, WA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corrie C.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"bibr1-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2021.101348"},{"key":"bibr2-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01998-16"},{"key":"bibr3-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.1998.0266"},{"key":"bibr4-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2021.151765"},{"key":"bibr5-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0540-z"},{"key":"bibr6-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1177\/10406387211047561"},{"key":"bibr7-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2018.2383"},{"key":"bibr8-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(98)00144-0"},{"key":"bibr9-10406387221126999","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2011.08.002"},{"key":"bibr10-10406387221126999","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8806"}],"container-title":["Journal of Veterinary Diagnostic Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221126999","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/10406387221126999","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/10406387221126999","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:26:02Z","timestamp":1777681562000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/10406387221126999"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,28]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1177\/10406387221126999"],"URL":"https:\/\/doi.org\/10.1177\/10406387221126999","relation":{},"ISSN":["1040-6387","1943-4936"],"issn-type":[{"value":"1040-6387","type":"print"},{"value":"1943-4936","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,28]]}}}