{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:29:20Z","timestamp":1790022560848,"version":"4.0.1"},"reference-count":17,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T00:00:00Z","timestamp":1680912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100017242","name":"National Institutes for Food and Drug Control","doi-asserted-by":"publisher","award":["2022C4"],"award-info":[{"award-number":["2022C4"]}],"id":[{"id":"10.13039\/100017242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:p>\n                    Hepatitis E virus (HEV) is a zoonotic pathogen that is a significant public health problem. Detecting HEV relies mainly on conventional PCR, which is time-consuming and requires sophisticated instruments and trained staff. We aimed to establish a reverse-transcription (RT)-recombinase polymerase amplification (RPA) assay (RT-RPA) combined with a lateral flow strip (LFS; RT-RPA-LFS) to rapidly detect HEV RNA in human and rabbit samples. With the optimal reaction conditions (37\u00b0C for 30\u2009min), our assay detected as few as 1.0\u2009\u00d7\u200910\n                    <jats:sup>2<\/jats:sup>\n                    copies\/mL of HEV and showed no cross-reactivity with other hepatitis viruses. We tested 28 human samples (4 fecal and 24 serum samples) and 360 rabbit samples (180 fecal and 180 serum samples) with our RT-RPA-LFS assay and compared our assay to an RT-qPCR method. There was no significant difference ( p\u2009&gt;\u20090.05) in the test results between the 2 assays. Our RT-RPA-LFS assay detected both HEV3 and HEV4 genotypes. Our rapid, sensitive, and specific RT-RPA-LFS assay for the detection of HEV may provide a useful detection tool for limited-resource areas.\n                  <\/jats:p>","DOI":"10.1177\/10406387231167119","type":"journal-article","created":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T07:25:21Z","timestamp":1680938721000},"page":"396-399","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Preliminary investigation of hepatitis E virus detection by a recombinase polymerase amplification assay combined with a lateral flow strip"],"prefix":"10.1177","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8679-1346","authenticated-orcid":false,"given":"Manyu","family":"Li","sequence":"first","affiliation":[{"name":"Division I of In Vitro Diagnostics for Infectious Diseases, Institute for In Vitro Diagnostics Control, National Institutes for Food and Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Hao","sequence":"additional","affiliation":[{"name":"Division I of In Vitro Diagnostics for Infectious Diseases, Institute for In Vitro Diagnostics Control, National Institutes for Food and Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyun","family":"Lan","sequence":"additional","affiliation":[{"name":"Division I of In Vitro Diagnostics for Infectious Diseases, Institute for In Vitro Diagnostics Control, National Institutes for Food and Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division I of In Vitro Diagnostics for Infectious Diseases, Institute for In Vitro Diagnostics Control, National Institutes for Food and Drug Control, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,4,8]]},"reference":[{"key":"bibr1-10406387231167119","doi-asserted-by":"publisher","DOI":"10.3201\/eid2307.170318"},{"key":"bibr2-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00135-13"},{"key":"bibr3-10406387231167119","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11030355"},{"key":"bibr4-10406387231167119","doi-asserted-by":"publisher","DOI":"10.3390\/v8100270"},{"key":"bibr5-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1111\/apt.14109"},{"key":"bibr6-10406387231167119","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v28.i1.76"},{"key":"bibr7-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.03.005"},{"key":"bibr8-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-024-0942-0_3"},{"key":"bibr9-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.86"},{"key":"bibr10-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1039\/C8AN01621F"},{"key":"bibr11-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2019.1643260"},{"key":"bibr12-10406387231167119","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12030621"},{"key":"bibr13-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.150"},{"key":"bibr14-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1929"},{"key":"bibr15-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a031880"},{"key":"bibr16-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-020-0175-x"},{"key":"bibr17-10406387231167119","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-024-0942-0_11"}],"container-title":["Journal 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