{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:02:03Z","timestamp":1791284523168,"version":"4.1.0"},"reference-count":19,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Multistate Conservation Grant Program","award":["F19AP00089"],"award-info":[{"award-number":["F19AP00089"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:p>The effects of filter paper strip (FPS) storage time and temperature on antibody detection are poorly understood despite widespread use in wildlife research. We collected sera and FPSs from 23 wild turkeys ( Meleagris gallopavo) and 20 northern bobwhites ( Colinus virginianus) experimentally infected with West Nile virus (WNV) to compare FPS storage methods with WNV plaque reduction neutralization test (PRNT) sensitivity. FPS storage methods included: immediate elution after drying, and storage at \u221220\u00b0C for 3\u2009mo, \u221220\u00b0C for 6\u2009mo, room temperature (RT) for 3\u2009mo, and RT for 6\u2009mo prior to elution. FPS eluates and sera were co-titrated to determine endpoint antibody titers, which were compared between FPS sera eluted immediately and sera, and among FPS eluates that underwent different storage conditions. Antibody titers were ~4-fold less in FPS sera eluted immediately versus sera, and dropped more frequently below PRNT detection threshold in northern bobwhites, which had ~10-fold lower serum antibody titers than wild turkeys. Antibody titers were lower in FPS samples stored at RT and for 6\u2009mo. WNV serologic surveys may result in falsely low seroprevalence estimates if FPSs are stored at RT for \u2265\u20093\u2009mo before elution.<\/jats:p>","DOI":"10.1177\/10406387231170795","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T05:32:56Z","timestamp":1682573576000},"page":"400-404","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Storage time and temperature of filter paper strips affect anti\u2013West Nile virus antibody detection in 2 galliform species"],"prefix":"10.1177","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-8393","authenticated-orcid":false,"given":"Melanie R.","family":"Kunkel","sequence":"first","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roy D.","family":"Berghaus","sequence":"additional","affiliation":[{"name":"Departments of Population Health, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Martin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, and Warnell School of Forestry and Natural Resources and Savannah River Ecology Laboratory, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel G.","family":"Mead","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark G.","family":"Ruder","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5931-175X","authenticated-orcid":false,"given":"Nicole M.","family":"Nemeth","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Wildlife Disease Study, University of Georgia, Athens, GA, USA"},{"name":"Pathology, University of Georgia, Athens, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"bibr1-10406387231170795","author":"Advantec","year":"2009","journal-title":"Toyo Roshi Kaisha"},{"key":"bibr2-10406387231170795","first-page":"204","volume-title":"Diagnostic Procedures for Viral, Rickettsial, and Chlamydial Infections","volume":"1995","author":"Beaty B","edition":"7"},{"key":"bibr3-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/2015-09-242"},{"key":"bibr4-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-47.1.12"},{"key":"bibr5-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/2012-02-047"},{"key":"bibr6-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/2012-06-150"},{"key":"bibr7-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1637\/9687-021511-ResNote.1"},{"key":"bibr8-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1002\/wsb.472"},{"key":"bibr9-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/JWD-D-20-00021"},{"key":"bibr10-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1637\/aviandiseases-D-21-00052"},{"key":"bibr11-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2022.2123732"},{"key":"bibr12-10406387231170795","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.310"},{"key":"bibr13-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-008-0183-z"},{"key":"bibr14-10406387231170795","doi-asserted-by":"publisher","DOI":"10.1177\/0300985817717770"},{"key":"bibr15-10406387231170795","doi-asserted-by":"publisher","DOI":"10.7589\/JWD-D-19-00016"},{"key":"bibr16-10406387231170795","doi-asserted-by":"publisher","DOI":"10.3390\/v6020752"},{"key":"bibr17-10406387231170795","unstructured":"R Core Team. 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