{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:10:12Z","timestamp":1787019012413,"version":"build-2736575974"},"reference-count":30,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100001250","name":"morris animal foundation","doi-asserted-by":"publisher","award":["D21CA-610"],"award-info":[{"award-number":["D21CA-610"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>Immunophenotyping of canine large-cell lymphoma (LCL) for B-cell and T-cell surface antigens is commonly performed to better predict the clinical outcome. Expression of surface antigen CD3 is associated with T-cell malignancies; surface antigen CD20 is expressed on B cells. However, a small subset of canine LCLs expresses both CD3 and CD20 (CD3+\/CD20+); this form of lymphoma remains poorly defined at the molecular level. In a retrospective study, we aimed to better characterize immunophenotypic properties and antigen receptor clonality of CD3+\/CD20+ LCL. We selected formalin-fixed, paraffin-embedded tissues from 10 cases of CD3+\/CD20+ LCL and breed-matched controls of peripheral large T-cell lymphoma (PTCL) and diffuse large B-cell lymphoma (DLBCL). Using PCR for antigen receptor rearrangement (PARR), we identified monoclonal T-cell receptor gamma (TCR\u03b3) rearrangements in all CD3+\/CD20+ cases. Three of 10 cases had monoclonal rearrangements in the immunoglobulin heavy chain (IgH), supportive of cross-lineage rearrangement. There was no significant difference in the frequency of antigen receptor rearrangement between CD3+\/CD20+ and PTCL cases. In comparison with DLBCL, CD3+\/CD20+ LCL had TCR\u03b3 rearrangement more frequently and IgH rearrangement less frequently, respectively. Immunolabeling of the B-cell marker PAX5 occurred less frequently in all CD3+\/CD20+ LCL cases compared to the DLBCL controls. Immunolabeling for BCL-2 was robust, regardless of immunophenotype. Nuclear Ki67 positivity was variable in CD3+\/CD20+ cases, indicating a heterogeneity in proliferation. Overall, cases of canine CD3+\/CD20+ LCL had properties similar to PTCL, suggesting a similar histogenesis of these 2 subsets.<\/jats:p>","DOI":"10.1177\/10406387231204873","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T02:10:12Z","timestamp":1697249412000},"page":"86-94","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of CD3+\/CD20+ canine large-cell lymphoma"],"prefix":"10.1177","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2582-8977","authenticated-orcid":false,"given":"Cory M.","family":"Howard","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation, Michigan State University College of Veterinary Medicine, East Lansing, MI, USA"},{"name":"Michigan State University Veterinary Diagnostic Laboratory, Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffanie","family":"Anderson","sequence":"additional","affiliation":[{"name":"Michigan State University Veterinary Diagnostic Laboratory, Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie","family":"Harrington","sequence":"additional","affiliation":[{"name":"Charles River Laboratories, Mattawan, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,10,13]]},"reference":[{"key":"bibr1-10406387231204873","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"bibr2-10406387231204873","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815604724"},{"key":"bibr3-10406387231204873","doi-asserted-by":"crossref","unstructured":"Brown EA, et al.\n                      FGF4\n                      retrogene on CFA12 is responsible for chondrodystrophy and intervertebral disc disease in dogs. 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