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However, no reports verify the autofluorescence of spontaneous A\u03b2 amyloidosis in animals, to our knowledge. We validated the autofluorescence of A\u03b2 lesions in spontaneous squirrel monkey cases under label-free conditions; lesions had intense blue-white autofluorescence in fluorescence microscopy using excitation light at 400\u2013440\u2009nm. Thioflavin S staining and immunohistochemistry of the same specimens revealed that this blue-white autofluorescence was derived from A\u03b2 lesions. Hyperspectral analysis of these lesions revealed a characteristic spectrum with bimodal peaks at 440 and 460\u2009nm, as reported for A\u03b2 lesions in mice. Principal component analysis using hyperspectral data specifically separated the A\u03b2 lesions from other autofluorescent substances, such as lipofuscin. A non-labeled and mechanistic detection of A\u03b2 lesions by hyperspectral imaging could provide valuable insights for developing early diagnostic techniques.<\/jats:p>","DOI":"10.1177\/10406387231204876","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T08:20:27Z","timestamp":1697185227000},"page":"41-45","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Label-free autofluorescence and hyperspectral imaging of cerebral amyloid-\u03b2 lesions in aged squirrel monkeys"],"prefix":"10.1177","volume":"36","author":[{"given":"Ryohei","family":"Oba","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Ujike","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Ono","sequence":"additional","affiliation":[{"name":"Advanced Technology Center, Corporate R&D Headquarters, Konica Minolta, Hachioji, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Okano","sequence":"additional","affiliation":[{"name":"Advanced Technology Center, Corporate R&D Headquarters, Konica Minolta, Hachioji, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5706-7842","authenticated-orcid":false,"given":"Tomoaki","family":"Murakami","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Cooperative Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,10,13]]},"reference":[{"key":"bibr1-10406387231204876","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63360-3"},{"key":"bibr2-10406387231204876","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-170665"},{"key":"bibr3-10406387231204876","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-019-00393-6"},{"key":"bibr4-10406387231204876","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12242-1"},{"key":"bibr5-10406387231204876","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.699024"},{"key":"bibr6-10406387231204876","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01981-x"},{"key":"bibr7-10406387231204876","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(97)00064-X"},{"key":"bibr8-10406387231204876","doi-asserted-by":"crossref","unstructured":"Murakami T, et al Failure of heterogeneous amyloid-enhancing factor in geriatric squirrel monkeys (\n                      Saimiri boliviensis\n                      ). 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