{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T21:41:46Z","timestamp":1777930906397,"version":"3.51.4"},"reference-count":22,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:00:00Z","timestamp":1722988800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"Equine Grass Sickness Fund","award":["John Gilmour Summer Research Scholarship"],"award-info":[{"award-number":["John Gilmour Summer Research Scholarship"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J VET Diagn Invest"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>Lipofuscin is a complex mixture of highly oxidized, cross-linked macromolecules that accumulates in neurons with age and some neurodegenerative diseases. Equine dysautonomia (ED) is a polyneuropathy that mainly affects autonomic and enteric nervous systems, resulting in alimentary tract dysfunction. Our main aim was to determine whether neuronal lipofuscin increased with increasing duration of ED. We investigated the prevalence of lipofuscin in cranial cervical ganglia of horses with acute (AED), subacute (SED), and chronic ED (CED), young controls (of similar age to ED cases), and aged controls ( n\u2009=\u20098\u2009per group). We used Schmorl stain for histologic detection of lipofuscin and assessed its accumulation in neurons using image analysis software. The percentage of neurons positive for lipofuscin increased with age in individual groups and all groups combined ( p\u2009&lt;\u20090.001). There were fewer positive neurons in AED and SED compared to aged controls ( p\u2009&lt;\u20090.001) and more in CED than AED cases ( p\u2009=\u20090.042) and young controls ( p\u2009=\u20090.012). We found a strong positive correlation between percentage positive neurons and percentage positive area of the neuron containing lipofuscin for combined groups ( p\u2009&lt;\u20090.001). Although neuronal lipofuscin increased in cranial cervical ganglion in CED cases, it remains to be determined whether this is a cause or consequence of neuronal degeneration.<\/jats:p>","DOI":"10.1177\/10406387241265715","type":"journal-article","created":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T02:54:53Z","timestamp":1723085693000},"page":"864-869","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Lipofuscin accumulates in ganglionic neurons in chronic equine dysautonomia"],"prefix":"10.1177","volume":"36","author":[{"given":"Lydia","family":"Tan Yi Shean","sequence":"first","affiliation":[{"name":"Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush Campus, Roslin, Midlothian, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4418-7746","authenticated-orcid":false,"given":"Elspeth 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